Li Xinyu, Ma Qianjie, Zheng Xiangtai, Chen Qin, Sun Xiaodong
Shanghai Engineering Research Center of Organ Repair, School of Medicine, Shanghai University, Shanghai, 200444, China.
Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
J Pharm Anal. 2023 Apr;13(4):323-339. doi: 10.1016/j.jpha.2023.01.003. Epub 2023 Feb 6.
Capillary electrochromatography (CEC) plays a significant role in chiral separation via the double separation principle, partition coefficient difference between the two phases, and electroosmotic flow-driven separation. Given the distinct properties of the inner wall stationary phase (SP), the separation ability of each SP differs from one another. Particularly, it provides large room for promising applications of open tubular capillary electrochromatography (OT-CEC). We divided the OT-CEC SPs developed over the past four years into six types: ionic liquids, nanoparticle materials, microporous materials, biomaterials, non-nanopolymers, and others, to mainly introduce their characteristics in chiral drug separation. There also added a few classic SPs that occurred within ten years as supplements to enrich the features of each SP. Additionally, we discuss their applications in metabolomics, food, cosmetics, environment, and biology as analytes in addition to chiral drugs. OT-CEC plays an increasingly significant role in chiral separation and may promote the development of capillary electrophoresis (CE) combined with other instruments in recent years, such as CE with mass spectrometry (CE/MS) and CE with ultraviolet light detector (CE/UV).
毛细管电色谱（CEC）通过双分离原理、两相之间的分配系数差异以及电渗流驱动分离在手性分离中发挥着重要作用。鉴于内壁固定相（SP）的独特性质，每种SP的分离能力各不相同。特别是，它为开管毛细管电色谱（OT-CEC）的应用提供了广阔的前景。我们将过去四年开发的OT-CEC SPs分为六种类型：离子液体、纳米颗粒材料、微孔材料、生物材料、非纳米聚合物和其他类型，主要介绍它们在手性药物分离中的特性。还增加了一些十年内出现的经典SPs作为补充，以丰富每种SP的特性。此外，除了手性药物外，我们还讨论了它们在代谢组学、食品、化妆品、环境和生物学中作为分析物的应用。OT-CEC在手性分离中发挥着越来越重要的作用，并且可能会促进近年来毛细管电泳（CE）与其他仪器的结合发展，如CE与质谱联用（CE/MS）以及CE与紫外光检测器联用（CE/UV）。
