Hirabayashi H, Koshii K, Uno K, Ohgaki H, Nakasone Y, Fujisawa T, Shono N, Hinohara T, Hirabayashi K
Department of Bronchoesophagology, Dokkyo University School of Medicine, Tochigi, Japan.
Auris Nasus Larynx. 1990;17(2):105-14. doi: 10.1016/s0385-8146(12)80192-1.
The human larynx is essential not only for speech, but also for swallowing and respiration. Its tissues are affected by the presence of alcohol and cigarette smoke. In this report we present our findings on the effects of smoking and drinking on histological changes in laryngeal tissue. The larynges studied were those of autopsy patients: cases with damaged tissue were excluded. We studied the larynges of 84 men (non-smoker and non-drinker, 22 cases; smoker and drinker, 62 cases) and 48 women (non-smoker and non-drinker, 40 cases; smoker and drinker, 8 cases) between the ages of 12 and 88. The squamous metaplasia of laryngeal epithelium was examined using the gross staining method (Pyronin Y) of STELL et al. (J. Laryngeal. Otol. 86: 589-594, 1972). The squamous epithelium is unstained, whereas the respiratory epithelium is a brilliant red. The area of metaplasia in the supraglottic region increased with aging, tobacco and alcohol consumption. Microscopic changes of the laryngeal epithelium were investigated using the hematoxylin-eosin staining method. The thickness of epithelium of the supraglottic region and vocal cord increased with age. There is a significant difference in the thickness of the epithelium of the supraglottic region and vocal cords of smoking and drinking patients, when compared with the same tissues of non-smokers and non-drinkers. Furthermore, there is a significant difference in those tissues of the supraglottic region of heavy smokers, when compared with light smokers, but no significant difference when compared with drinkers. However, there is no significant difference in the thickness of vocal cord tissues when comparing light smokers and drinkers on the one hand, and heavy smokers and drinkers on the other.
人类喉部不仅对言语至关重要，对吞咽和呼吸也必不可少。其组织会受到酒精和香烟烟雾的影响。在本报告中，我们展示了关于吸烟和饮酒对喉部组织组织学变化影响的研究结果。所研究的喉部来自尸检患者：排除了组织受损的病例。我们研究了12至88岁的84名男性（非吸烟者和非饮酒者，22例；吸烟者和饮酒者，62例）以及48名女性（非吸烟者和非饮酒者，40例；吸烟者和饮酒者，8例）的喉部。采用斯特尔等人（《喉科学与耳科学杂志》86: 589 - 594, 1972）的大体染色方法（派若宁Y）检查喉上皮的鳞状化生。鳞状上皮不着色，而呼吸上皮呈鲜红色。声门上区化生面积随年龄、烟草和酒精摄入量增加而增大。使用苏木精 - 伊红染色方法研究喉上皮的微观变化。声门上区和声带的上皮厚度随年龄增加。与非吸烟者和非饮酒者的相同组织相比，吸烟和饮酒患者的声门上区和声带上皮厚度存在显著差异。此外，重度吸烟者声门上区的那些组织与轻度吸烟者相比存在显著差异，但与饮酒者相比无显著差异。然而，一方面比较轻度吸烟者和饮酒者，另一方面比较重度吸烟者和饮酒者时，声带组织厚度无显著差异。
