Qu Jialin, Gong Tianxing, Ma Bin, Zhang Lin, Kano Yoshihiro, Yuan Dan
School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang 110016, China.
Chem Pharm Bull (Tokyo). 2012;60(1):23-30. doi: 10.1248/cpb.60.23.
The purpose of the study is to compare alkaloid profile of Uncaria rhynchophylla hooks and leaves. Ten oxindole alkaloids and four glycosidic indole alkaloids were identified using HPLC-diode array detection (DAD) or LC-atmospheric pressure chemical ionization (APCI)-MS method, and a HPLC-UV method for simultaneous quantification of major alkaloids was validated. The hooks are characterized by high levels of four oxindole alkaloids rhynchophylline (R), isorhynchophylline (IR), corynoxeine (C) and isocorynoxeine (IC), while the leaves contained high level of two glycosidic indole alkaloids vincoside lactam (VL) and strictosidine (S). The presented methods have proven its usefulness in chemical characterization of U. rhynchophylla hooks and leaves.
本研究的目的是比较钩藤的钩和叶中的生物碱谱。使用高效液相色谱-二极管阵列检测（DAD）或液相色谱-大气压化学电离（APCI）-质谱法鉴定了10种氧化吲哚生物碱和4种糖苷吲哚生物碱，并验证了一种用于同时定量主要生物碱的高效液相色谱-紫外法。钩的特征是含有高水平的四种氧化吲哚生物碱，即钩藤碱（R）、异钩藤碱（IR）、柯诺辛碱（C）和异柯诺辛碱（IC），而叶中含有高水平的两种糖苷吲哚生物碱，即长春苷内酰胺（VL）和裂环马钱子苷（S）。所提出的方法已证明其在钩藤的钩和叶的化学表征中的有用性。
