Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, Santa Maria, Rio Grande do Sul, Brazil.
Cell Biochem Funct. 2012 Jun;30(4):286-92. doi: 10.1002/cbf.2797. Epub 2012 Jan 6.
α-Tocopherol (α-Toc) is involved in various physiologic processes, which present antioxidant and neuroprotective properties. High-fat diets have an important role in neurodegenerative diseases and neurological disturbances. This study aimed to investigate the effects of treatment with α-Toc and the consumption of high-fat diets on ectonucleotidase activities in synaptosomes of cerebral cortex, hippocampus and striatum of rats. Animals were divided into four different groups, which received standard diet (control), high-fat saturated diet (HF), α-Toc and high-fat saturated diet plus α-Toc (α-Toc + HF). High-fat saturated diet was administered ad libitum and α-Toc by gavage using a dose of 50 mg·kg(-1). After 3 months of treatment, animals were submitted to euthanasia, and cerebral cortex, hippocampus and striatum were collected for biochemical assays. Results showed that adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) hydrolysis in the cerebral cortex, hippocampus and striatum were decreased in HF in comparison to the other groups (P < 0·05). When rats that received HF were treated with α-Toc, the activity of the ectonucleotidases was similar to the control. ATP, ADP and AMP hydrolysis in the cerebral cortex, hippocampus and striatum were increased in the α-Toc group when compared with the other groups (P < 0·05). These findings demonstrated that the HF alters the purinergic signaling in the nervous system and that the treatment with α-Toc was capable of modulating the adenine nucleotide hydrolysis in this experimental condition.
α-生育酚（α-Toc）参与各种生理过程，具有抗氧化和神经保护特性。高脂肪饮食在神经退行性疾病和神经功能障碍中起着重要作用。本研究旨在探讨 α-生育酚治疗和高脂肪饮食对大鼠大脑皮质、海马和纹状体突触体中外核苷酸酶活性的影响。动物分为四组，分别给予标准饮食（对照组）、高脂肪饱和饮食（HF）、α-生育酚和高脂肪饱和饮食加 α-生育酚（α-Toc+HF）。高脂肪饱和饮食自由摄取，α-生育酚通过灌胃给药，剂量为 50mg·kg(-1)。治疗 3 个月后，动物安乐死，采集大脑皮质、海马和纹状体进行生化分析。结果显示，与其他组相比，HF 组大脑皮质、海马和纹状体中的三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和一磷酸腺苷（AMP）水解均降低（P<0.05）。当接受 HF 的大鼠用 α-生育酚治疗时，外核苷酸酶的活性与对照组相似。与其他组相比，α-Toc 组大脑皮质、海马和纹状体中的 ATP、ADP 和 AMP 水解增加（P<0.05）。这些发现表明 HF 改变了神经系统中的嘌呤能信号，并且 α-生育酚治疗能够调节这种实验条件下的腺嘌呤核苷酸水解。
