克氏锥虫组蛋白。进一步的特征分析及与高等真核生物的比较。

Trypanosoma cruzi histones. Further characterization and comparison with higher eukaryotes.

作者信息

Toro G C, Galanti N

机构信息

Departamento de Biología Celular y Genética, Facultad de Medicina, Universidad de Chile, Santiago.

出版信息

Biochem Int. 1990;21(3):481-90.

PMID:2222493

Abstract

Histones extracted from T. cruzi chromatin were analyzed in three electrophoretic systems. Our results show that a basic protein with some properties similar to those of histone H1 from higher eukaryotes is present in T. cruzi. However this protein presents different electrophoretic mobilities than H1 histone from higher eukaryotes in all three electrophoretic systems tested. Considering the marked differences observed in the electrophoretic mobilities of T. cruzi histones as compared with those from higher eukaryotes, it is proposed that histones are conservative proteins primarily with regard to their function.

摘要

对从克氏锥虫染色质中提取的组蛋白在三种电泳系统中进行了分析。我们的结果表明，克氏锥虫中存在一种碱性蛋白，其某些特性与高等真核生物的组蛋白H1相似。然而，在所有测试的三种电泳系统中，这种蛋白的电泳迁移率与高等真核生物的H1组蛋白不同。考虑到与高等真核生物相比，克氏锥虫组蛋白在电泳迁移率上存在显著差异，有人提出组蛋白主要在功能方面是保守蛋白。