Marzotto Marta, Conforti Anita, Magnani Paolo, Zanolin Maria Elisabetta, Bellavite Paolo
Department of Pathology and Diagnostics, University of Verona (I), Italy.
Homeopathy. 2012 Jan;101(1):57-67. doi: 10.1016/j.homp.2011.10.001.
Ignatia amara (Ignatia), a remedy made from the Strychnos ignatii seeds, is used for anxiety-related symptoms, but consistent evidence of its activity in reproducible experimental models is lacking. An investigation was performed in order to assess on mice, by means of emotional response models, the activity of homeopathic Ignatia dilutions/dynamizations.
Groups of 8 mice of the CD1 albino strain were treated intraperitoneally for 9 days with 0.3ml of five centesimal (C) dilutions/dynamizations of Ignatia (4C, 5C, 7C, 9C and 30C). Control mice were treated with the same hydroalcoholic (0.3%) solution used to dilute the medicines. Diazepam (1mg/kg) was the positive reference drug. Validated test models for locomotion and emotional response, the Open-Field (OF) and the Light-Dark (LD) tests, were employed. Five replications of the same protocol were carried out, in a randomised way using coded drugs/controls.
In the OF the general locomotion of mice was slightly decreased by Ignatia 4C, but not by Ignatia 5C, 7C, 9C and 30C, indicating the absence of unspecific motor impairment or sedation by these dilutions/dynamizations. Ignatia and diazepam seemed to decrease the number of urine spots released in the OF during 10min, with borderline significance (P=0.083). In the LD the tested medicine showed anxiolytic-like activity (increase of time spent and distance travelled in the lit area), though to a lesser extent than diazepam. The highest and most significant difference with untreated controls (P<0.01) was observed with the 9C dilution/dynamization. Among the 5 replication experiments, the best drug effects were obtained where the baseline anxiety of mice was higher.
Homeopathic Ignatia dilutions/dynamizations (peak at 9C) modify some emotion-related symptoms in laboratory mice without affecting locomotion.
阿姆拉橙花（Ignatia）是一种由马钱子种子制成的药物，用于治疗与焦虑相关的症状，但在可重复的实验模型中缺乏其活性的一致性证据。为了通过情绪反应模型评估顺势疗法的阿姆拉橙花稀释液/增效剂对小鼠的活性，开展了一项研究。
将8只CD1白化病品系小鼠分为一组，腹腔注射0.3ml阿姆拉橙花的五种百分之一（C）稀释液/增效剂（4C、5C、7C、9C和30C），持续9天。对照小鼠用用于稀释药物的相同水醇（0.3%）溶液进行处理。地西泮（1mg/kg）为阳性对照药物。采用经过验证的运动和情绪反应测试模型，即旷场试验（OF）和明暗试验（LD）。使用编码药物/对照以随机方式对相同方案进行了五次重复实验。
在旷场试验中，阿姆拉橙花4C使小鼠的总体运动略有下降，但5C、7C、9C和30C则没有，表明这些稀释液/增效剂不存在非特异性运动损伤或镇静作用。阿姆拉橙花和地西泮似乎减少了10分钟内在旷场试验中释放的尿斑数量，具有临界显著性（P=0.083）。在明暗试验中，受试药物显示出抗焦虑样活性（在光照区域停留时间和行进距离增加），尽管程度低于地西泮。9C稀释液/增效剂与未处理对照相比差异最大且最显著（P<0.01）。在5次重复实验中，在小鼠基线焦虑较高的情况下获得了最佳药物效果。
顺势疗法的阿姆拉橙花稀释液/增效剂（在9C时达到峰值）可改变实验室小鼠中一些与情绪相关的症状，而不影响运动。
