Hall David H, Hartwieg Erika, Nguyen Ken C Q
Center for C. elegans Anatomy, Albert Einstein College of Medicine, Bronx, New York, USA.
Methods Cell Biol. 2012;107:93-149. doi: 10.1016/B978-0-12-394620-1.00004-7.
From its inception as a model organism 40 years ago, Caenorhabditis elegans was chosen in part for its suitability for study in serial thin sections by electron microscopy. Recent improvements in electron microscopy technology are making this pursuit more reliable and more powerful. In this chapter, we highlight new methods in specimen preparation, imaging, and data analysis. Accurate three-dimensional information can now be obtained for the whole animal at all stages, down to the level of individual organelles and the cytoskeleton.
40年前秀丽隐杆线虫作为一种模式生物被确立，部分原因是它适合通过电子显微镜进行连续超薄切片研究。电子显微镜技术的最新进展使这一研究更加可靠且更具威力。在本章中，我们重点介绍标本制备、成像和数据分析方面的新方法。现在可以在各个阶段获取整个动物直至单个细胞器和细胞骨架水平的准确三维信息。
