Hall David H, Rice William J
Albert Einstein College of Medicine, Center for C. elegans Anatomy, 1410 Pelham Parkway South, Room 601, Bronx, NY, 10461, USA.
New York Structural Biology Center, New York, NY, USA.
Methods Mol Biol. 2015;1327:141-58. doi: 10.1007/978-1-4939-2842-2_11.
Methods for electron tomography of the nematode C. elegans are explained in detail, including a brief introduction to specimen preparation, methods for image collection, and a comparison of several general methods for producing dual-axis tomograms, with or without external fiducial reference objects. New electron tomograms highlight features in software for data display, annotation, and analysis. This chapter discusses the ultrastructural analysis of cells and tissues, rather than molecular studies.
