Bossinger Olaf, Cowan Carrie R
MOCA, RWTH Aachen, 52074 Aachen Germany.
Methods Cell Biol. 2012;107:207-38. doi: 10.1016/B978-0-12-394620-1.00007-2.
Cell polarity is a fundamental principle guiding development. Early C. elegans embryos contain a diversity of polarized cell types, ranging from asymmetrically dividing stem cells to a polarized epithelium. Over the past two decades, work using C. elegans embryos has led to mechanistic understandings of many aspects of cell polarity establishment, maintenance and propagation. Here we provide basic methods for researchers interested in using C. elegans embryos to study cell polarity, emphasizing the amenability of C. elegans to quantitative molecular analysis.
细胞极性是指导发育的一项基本原理。早期秀丽隐杆线虫胚胎包含多种极化细胞类型，从不对称分裂的干细胞到极化上皮细胞。在过去二十年中，利用秀丽隐杆线虫胚胎开展的研究工作已使人们对细胞极性建立、维持和传播的诸多方面有了机制上的理解。在此，我们为有兴趣利用秀丽隐杆线虫胚胎研究细胞极性的研究人员提供基本方法，着重强调秀丽隐杆线虫适用于定量分子分析。
