Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea.
Vet Parasitol. 2012 Jun 8;187(1-2):147-56. doi: 10.1016/j.vetpar.2011.12.006. Epub 2011 Dec 19.
Olive flounder, Paralichythys olivaceus fed with pomegranate enriched diet and challenged with or without Philasterides dicentrarchi had a significantly higher white blood cell (WBC) count on weeks 2 and 4 than the infected group fed with non enriched diet (standard diet). Similarly the red blood cell (RBC) counts did not significantly change in control and treated fish on weeks 1 and 2. It was significantly increased in treated fish on week 2 when compared to the control. In both the groups the hemoglobin (Hb) and hematocrit (Ht) levels significantly increased on weeks 2 and 4. The mean corpuscular volume (MCV) did not significantly change at any time in both groups whereas mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) increased significantly on week 4 in the treated group. The leukocytes such as lymphocytes (Lym), monocytes (Mon), neutrophils (Neu), and biochemical parameters such as total protein (TP), glucose (GLU), and calcium (CAL) levels significantly increased in treated groups on week 2 or 4 as compared to the control. The scuticocidal activity and respiratory burst activity were significantly enhanced in treated groups with or without parasite on weeks 2-4. However, the serum lysozyme activity was significantly enhanced from weeks 1 to 4. The protective response in terms of cumulative mortality was low in groups fed with enriched diet against parasite when compared to control. Therefore, we suggest that pomegranate enriched diet following challenge with P. dicentrarchi restores the altered hematological and biochemical parameters, and improves the innate immune system in olive flounder against P. dicentrarchi.
投喂富含石榴的饲料并同时感染和不感染 Philasterides dicentrarchi 的橄榄石斑鱼，在第 2 周和第 4 周的白细胞（WBC）计数显著高于未感染且未投喂富含石榴饲料的感染组（标准饲料）。同样，在第 1 周和第 2 周，对照组和治疗组的红细胞（RBC）计数没有显著变化。与对照组相比，治疗组在第 2 周的 RBC 计数显著增加。在这两组中，血红蛋白（Hb）和红细胞压积（Ht）水平在第 2 周和第 4 周均显著升高。在这两组中，平均红细胞体积（MCV）在任何时间都没有显著变化，而平均红细胞血红蛋白（MCH）和平均红细胞血红蛋白浓度（MCHC）在治疗组第 4 周显著增加。白细胞如淋巴细胞（Lym）、单核细胞（Mon）、嗜中性粒细胞（Neu）和生化参数如总蛋白（TP）、葡萄糖（GLU）和钙（CAL）水平在第 2 周或第 4 周的治疗组中与对照组相比显著增加。在第 2-4 周，无论是否有寄生虫，治疗组的杀虫活性和呼吸爆发活性均显著增强。然而，血清溶菌酶活性从第 1 周到第 4 周显著增强。与对照组相比，投喂富含石榴的饲料对寄生虫的累积死亡率保护反应较低。因此，我们认为，在受到 P. dicentrarchi 挑战后，投喂富含石榴的饲料可以恢复橄榄石斑鱼的血液学和生化参数的改变，并改善其先天免疫系统。
