Department of Biotechnology and Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg, Germany.
J Struct Biol. 2012 Feb;177(2):344-8. doi: 10.1016/j.jsb.2011.12.015. Epub 2011 Dec 29.
Super-resolution microscopy is a powerful tool for understanding cellular function. However one of the most important biomolecules - DNA - remains somewhat inaccessible because it cannot be effectively and appropriately labeled. Here, we demonstrate that robust and detailed super-resolution images of DNA can be produced by combining 5-ethynyl-2'-deoxyuridine (EdU) labeling using the 'click chemistry' approach and direct stochastic optical reconstruction microscopy (dSTORM). This method can resolve fine chromatin structure, and - when used in conjunction with pulse labeling - can reveal the paths taken by individual fibers through the nucleus. This technique should provide a useful tool for the study of nuclear structure and function.
超分辨率显微镜是理解细胞功能的有力工具。然而，其中最重要的生物分子之一——DNA——仍然有些难以接近，因为它不能被有效地、适当地标记。在这里，我们通过结合“点击化学”方法和直接随机光学重建显微镜（dSTORM）对 5-乙炔基-2'-脱氧尿苷（EdU）标记进行使用，证明可以产生稳健且详细的 DNA 超分辨率图像。该方法可以解析精细的染色质结构，并且-当与脉冲标记结合使用时-可以显示单个纤维穿过核的路径。该技术应为研究核结构和功能提供有用的工具。
