Brain Science Institute, RIKEN, Wako-city, Saitama, Japan.
Biochem Biophys Res Commun. 2012 Jan 20;417(3):1080-5. doi: 10.1016/j.bbrc.2011.12.100. Epub 2011 Dec 27.
We have developed a multi-target cell tracking program TADOR, which we applied to a series of fluorescence images. TADOR is based on an active contour model that is modified in order to be free of the problem of locally optimal solutions, and thus is resistant to signal fluctuation and morphological changes. Due to adoption of backward tracing and addition of user-interactive correction functions, TADOR is used in an off-line and semi-automated mode, but enables precise tracking of cell division. By applying TADOR to the analysis of cultured cells whose nuclei had been fluorescently labeled, we tracked cell division and cell-cycle progression on coverslips over an extended period of time.
我们开发了一个多目标细胞跟踪程序 TADOR，我们将其应用于一系列荧光图像。TADOR 基于一个主动轮廓模型，该模型经过修改，以避免局部最优解决方案的问题，因此能够抵抗信号波动和形态变化。由于采用了回溯跟踪和添加用户交互修正功能，TADOR 以离线和半自动模式使用，但能够精确跟踪细胞分裂。通过将 TADOR 应用于经荧光标记的细胞核的培养细胞的分析，我们在较长时间内跟踪盖玻片上的细胞分裂和细胞周期进程。
