Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, CP 1686, Hurlingham, Buenos Aires, Argentina.
J Virol Methods. 2010 Aug;167(2):152-7. doi: 10.1016/j.jviromet.2010.03.026. Epub 2010 Mar 31.
The purpose of this study was to develop and evaluate a simple immunochromatographic lateral flow (ICLF) test for specific detection of Equine infectious anemia virus (EIAV) antibodies in equine sera. Viral recombinant p26 capsid protein (rp26) was used as the capture protein in the test line and as the detector reagent conjugated to colloidal gold. The performance of rp26-ICLF was evaluated, and the results obtained were compared with a commercially available agar gel immunodiffusion (AGID) test used as a standard of comparison according to international guidelines. The values obtained for comparative diagnostic sensitivity (98.3%), diagnostic specificity (87.4%) and concordance (92.4%) were similar to those reported for other ICLF tests for animal infectious diseases. Very good repeatability and reproducibility, as well as a total agreement with blind previous results from three proficiency test panels, were obtained, thus indicating that rp26-ICLF is a precise test. The end point of the twofold serial dilution of serum samples was the same as, and even better than, the AGID test, thus demonstrating the same analytical sensitivity as that of the reference method for EIA diagnosis. No cross-reactivity was observed when serum samples from horses with other infectious diseases were analyzed. rp26-ICLF proved to be a precise and rapid test suitable for field screening in veterinary practice, since minimal equipment and operator expertise are required. However, further research should be carried out to increase the level of sensitivity.
本研究旨在开发和评估一种简单的免疫胶体金层析（ICLF）检测方法，用于检测马血清中的马传染性贫血病毒（EIAV）抗体。病毒重组 p26 衣壳蛋白（rp26）被用作测试线中的捕获蛋白，并作为与胶体金偶联的检测试剂。评估了 rp26-ICLF 的性能，并根据国际指南，将与商业上可用的琼脂凝胶免疫扩散（AGID）测试的结果进行比较。比较诊断敏感性（98.3%）、诊断特异性（87.4%）和一致性（92.4%）的结果与其他动物传染病的 ICLF 检测相似。该检测具有非常好的重复性和重现性，与三个能力验证小组的盲法先前结果完全一致，表明 rp26-ICLF 是一种精确的检测方法。血清样品的两倍系列稀释终点与 AGID 检测相同，甚至更好，因此表明其与 EIA 诊断的参考方法具有相同的分析灵敏度。当分析来自患有其他传染病的马的血清样本时，未观察到交叉反应。rp26-ICLF 被证明是一种精确且快速的检测方法，适用于兽医实践中的现场筛选，因为它所需的设备和操作人员的专业知识很少。然而，应该进行进一步的研究以提高灵敏度水平。
