Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Spain.
Anal Bioanal Chem. 2012 Mar;402(7):2369-78. doi: 10.1007/s00216-011-5660-5. Epub 2012 Jan 7.
Perfluorinated compounds (PFCs) are ubiquitous contaminants of humans and animals worldwide. PFCs are bioaccumulated because of their affinity for proteins. It has been shown they could have a variety of toxicological effects and cause damage to human health, emphasizing the need for sensitive and robust analytical methods to assess their bioaccumulation in humans. In this paper we report the development and validation of an analytical method for analysis of PFCs in the non-invasive human matrices hair and urine. The method is based on rapid and simple sample pre-treatment followed by online turbulent flow liquid chromatography and tandem mass spectrometry (TFC-LC-MS-MS) for analysis of 21 PFCs. The method was validated for both matrices. Percentage recovery was between 60 and 105 for most compounds in both matrices. Limits of quantification ranged from 0.1 to 9 ng mL(-1) in urine and from 0.04 to 13.4 in hair. The good performance of the method was proved by investigating the presence of selected PFCs in 24 hair and 30 urine samples from different donors living in Barcelona (NE Spain). The results were indicative of bioaccumulation of these compounds in both types of sample. PFOS and PFOA were most frequently detected in hair and PFBA in urine.
全氟化合物（PFCs）是全球范围内人类和动物普遍存在的污染物。由于其与蛋白质的亲和力，PFCs 具有很强的生物蓄积性。已证明它们可能具有多种毒理学效应，并对人类健康造成损害，这强调了需要使用灵敏且强大的分析方法来评估其在人体中的生物蓄积情况。本文报告了一种用于分析非侵入性人体基质头发和尿液中 PFCs 的分析方法的开发和验证。该方法基于快速而简单的样品预处理，随后进行在线湍流流动液相色谱和串联质谱（TFC-LC-MS-MS）分析 21 种 PFCs。该方法在两种基质中均得到了验证。大多数化合物在两种基质中的回收率在 60%至 105%之间。尿液中的定量限范围为 0.1 至 9ng/mL，头发中的定量限范围为 0.04 至 13.4ng/mL。通过调查来自西班牙东北部巴塞罗那的不同供体的 24 份头发和 30 份尿液样本中选定 PFCs 的存在情况，证明了该方法的良好性能。结果表明这些化合物在这两种类型的样本中均有生物蓄积。在头发中最常检测到 PFOS 和 PFOA，在尿液中最常检测到 PFBA。
