Kremers Jan, Jertila Mounira, Link Barbara, Pangeni Gobinda, Horn Folkert K
Department of Ophthalmology, University Hospital Erlangen, Erlangen, Germany.
Doc Ophthalmol. 2012 Apr;124(2):79-90. doi: 10.1007/s10633-011-9304-z. Epub 2012 Jan 8.
Flash electroretinogram responses were measured in normal subjects to different chromatic combinations of flashes and backgrounds. The amplitudes of the flash response components were measured at different flash strengths and could be described by a generalized Naka-Rushton function. The measurements were repeated at different background luminances to study adaptation effects. It was found that when flash strength and background luminance were expressed in photometric terms (cd s/m² and cd/m², respectively), then the responses were very similar for all chromatic combinations with the exception of the condition in which blue (peak wavelength 458 nm) was flashed upon an orange (peak wavelength 591 nm) background. We propose that in this condition, a second (possibly S-cone or rod-driven) mechanism intrudes. The negative response after the b-wave (here called "photopic negative response" or PhNR for all conditions) is thought to reflect ganglion cell activity and was also largest at this condition. Responses were measured to the 458 nm flash on 591 nm background and the reversed combination in a population of 39 normal subjects and 49 glaucoma patients. It was found that the PhNR amplitude was affected by glaucoma in all conditions. Other component parameters, reflecting responses and adaptation dynamics, were not altered. The best stimulus condition among the conditions used to separate the PhNR amplitude of normals and patients was a 1 cd s/m² 458 nm flash on a 10 cd/m² 591 nm background.
在正常受试者中测量了闪光视网膜电图对不同闪光和背景颜色组合的反应。在不同的闪光强度下测量闪光反应成分的振幅，这些振幅可用广义的中谷-拉什顿函数来描述。在不同的背景亮度下重复测量以研究适应效应。结果发现，当闪光强度和背景亮度用光度学术语表示（分别为cd s/m²和cd/m²）时，除了在橙色（峰值波长591 nm）背景上闪现蓝色（峰值波长458 nm）的情况外，所有颜色组合的反应都非常相似。我们认为在这种情况下，第二种（可能由S视锥细胞或视杆细胞驱动）机制介入。b波后的负反应（在所有情况下这里称为“明视负反应”或PhNR）被认为反映神经节细胞的活动，并且在这种情况下也是最大的。在39名正常受试者和49名青光眼患者中测量了对591 nm背景上458 nm闪光以及相反组合的反应。结果发现，在所有情况下PhNR振幅都受青光眼影响。反映反应和适应动态变化的其他成分参数没有改变。用于区分正常人和患者PhNR振幅的最佳刺激条件是在10 cd/m²的591 nm背景上施加1 cd s/m² 的458 nm闪光。
