Department of Anesthesiology, FuWai Cardiovascular Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beilishi Road, Xicheng District, Beijing, 100037, People's Republic of China.
Mol Biol Rep. 2012 May;39(5):6049-57. doi: 10.1007/s11033-011-1419-5. Epub 2012 Jan 7.
Sevoflurane postconditioning is a potential clinical measure to protect myocardial. This experiment was designed to investigate the efficacy of sevoflurane postconditioning against ischemia-reperfusion injury. A total of 132 Japanese White Rabbits were enrolled into this study. They were underwent 15-, 30-, or 60-min left anterior descending coronary (LAD) artery occlusion, respectively. At the end of LAD artery occlusion, they randomly received a 5-min inhalation of air (control group), 1% sevoflurane (1% sev group), 2% sevoflurane (2% sev group), 4% sevoflurane (4% sev group) or an IV bolus injection of 5 mg/kg of NIM811 [a specific inhibitor of mitochondrial permeability transition pores (mPTP)]. Infarct size was determined after 2 h of reperfusion (triphenyltetrazolium chloride straining, percentage of risk area). The infarct sizes were significantly (P < 0.05) reduced after 15 min ischemia (5.5 ± 3.3%, 5.8 ± 3.6% vs. 20.3 ± 6.9% for 2% sev, 4% sev vs. control, respectively) and 30 min ischemia (23.5 ± 5.0%, 20.7 ± 5.9% vs. 50.9 ± 10.2%, for 2% sev, 4% sev vs. control, respectively; P < 0.05). However, it had no effect on infarct size after 60 min ischemia (64.1 ± 5.9%, 62.3 ± 7.6% vs. 72.7 ± 9.2% for 2% sev, 4% sev vs. control, respectively, P > 0.05).The efficacy of sevoflurane postconditioning gradually weakened with increasing ischemia duration and disappears after 60 min ischemia in rabbits in vivo.
七氟醚后处理是一种保护心肌的潜在临床措施。本实验旨在研究七氟醚后处理对缺血再灌注损伤的疗效。共纳入 132 只日本大白兔，分别行 15、30 或 60min 左前降支（LAD）动脉闭塞。在 LAD 动脉闭塞结束时，它们随机接受 5min 空气吸入（对照组）、1%七氟醚（1% sev 组）、2%七氟醚（2% sev 组）、4%七氟醚（4% sev 组）或 5mg/kg NIM811（一种线粒体通透性转换孔（mPTP）的特异性抑制剂）静脉推注。再灌注 2h 后测定梗死面积（氯化三苯基四氮唑染色，危险区百分比）。15min 缺血后（2% sev、4% sev 组与对照组相比分别为 5.5±3.3%、5.8±3.6%和 20.3±6.9%）和 30min 缺血后（2% sev、4% sev 组与对照组相比分别为 23.5±5.0%、20.7±5.9%和 50.9±10.2%）梗死面积明显减少（P<0.05）。然而，60min 缺血后，它对梗死面积没有影响（2% sev、4% sev 组与对照组相比分别为 64.1±5.9%、62.3±7.6%和 72.7±9.2%）（P>0.05）。七氟醚后处理的疗效随缺血时间的延长而逐渐减弱，在兔体内 60min 缺血后消失。
