First Department of Pathology, Medical School, University of Athens, Greece.
APMIS. 2012 Feb;120(2):92-100. doi: 10.1111/j.1600-0463.2011.02814.x. Epub 2011 Sep 22.
The aim of our study was to investigate the expression of Smad-7 and Ski proteins in invasive breast carcinomas, to determine their clinicopathological value and their influence on carcinomas biologic behavior. Immunohistochemistry was applied on 150 invasive breast carcinomas to detect the expression of Smad-7 and Ski. Their correlation to clinicopathologic parameters and markers of metastasis was statistically processed using chi-squared test. Overall and disease-free survival was assessed using Kaplan-Meier test and log-rank statistics. Smad-7 was immunodetected in the cytoplasm of cancer cells in 60%, whereas Ski was immunodetected in the cytoplasm and nuclei in 44.5% and 17.6% of the cases, respectively. Smad-7 expression was positively correlated with tumor size, stage, matrix metalloproteinase (MMP)-9, and MMP-14. Cytoplasmic Ski expression was negatively associated with tumor size, stage, and lymph node status, and its nuclear expression was negatively related to histologic grade. Cytoplasmic Ski expression was associated with longer overall and disease-free survival. It appears that two negative regulators of the transforming growth factor-β pathway, Smad-7 and Ski, behave differentially in invasive breast carcinomas. Smad-7 appears to be related with an aggressive phenotype, whereas Ski expression is related to a less aggressive behavior and positively influences patients' survival.
我们的研究目的是探讨 Smad-7 和 Ski 蛋白在浸润性乳腺癌中的表达，以确定其临床病理价值及其对癌生物学行为的影响。应用免疫组织化学方法检测了 150 例浸润性乳腺癌中 Smad-7 和 Ski 的表达。使用卡方检验对它们与临床病理参数和转移标志物的相关性进行了统计学处理。采用 Kaplan-Meier 检验和对数秩检验评估总生存和无病生存。Smad-7 在 60%的癌细胞细胞质中被免疫检测到，而 Ski 在细胞质和细胞核中分别被免疫检测到 44.5%和 17.6%的病例。Smad-7 的表达与肿瘤大小、分期、基质金属蛋白酶（MMP）-9 和 MMP-14 呈正相关。细胞质 Ski 的表达与肿瘤大小、分期和淋巴结状态呈负相关，其核表达与组织学分级呈负相关。细胞质 Ski 的表达与总生存和无病生存时间延长相关。两种转化生长因子-β通路的负性调节因子 Smad-7 和 Ski 在浸润性乳腺癌中的表现不同。Smad-7 似乎与侵袭性表型相关，而 Ski 的表达与侵袭性较低的行为相关，并对患者的生存产生积极影响。
