Departments of Biochemistry & Molecular Biology and Pediatrics, College of Medicine, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, AR 72205, USA.
Transfusion. 2012 May;52(5):963-73. doi: 10.1111/j.1537-2995.2011.03512.x. Epub 2012 Jan 9.
Measurement of red blood cell (RBC) survival (RCS) is important for investigating pathophysiology and treatment of anemia. Our objective was to validate the multidensity biotin method for RCS determination in sheep, a commonly used model of RBC physiology. [(14) C]Cyanate served as the reference method for long-term RCS because the (51) Cr method (the reference method for humans) is not reliable in sheep.
Aliquots of autologous RBCs from eight adult sheep were labeled with [(14) C]cyanate and four separate densities of biotin (BioRBCs) and reinfused. Short-term RCS was assessed by posttransfusion recovery at 24 hours (PTR(24) ); long-term RCS was assessed by the time to 50% survival (T(50) ) and mean potential life span (MPL).
Values for PTR(24) of the four BioRBC densities were not different. Values for RCS as reflected by T(50) and MPL were nearly identical for [(14) C]cyanate and the two intermediate-density BioRBC populations. In contrast, the lowest-density BioRBC population survived slightly longer (p < 0.01), but with a difference of no clinical significance. The highest-density BioRBC population importantly shortened RCS (p < 0.01 compared to the two intermediate densities).
This study provides evidence that BioRBCs labeled at four biotin densities can be used to independently and simultaneously measure short-term RCS and that BioRBCs labeled at the three lowest biotin densities can be used to accurately and simultaneously measure long-term RCS. Because the sheep RBC model is comparable to humans, this nonradioactive method has promise for use in RBC kinetic studies in neonates and pregnant women.
红细胞（RBC）生存力（RCS）的测量对于研究贫血的病理生理学和治疗方法非常重要。我们的目的是验证用于绵羊 RBC 生理学研究的多密度生物素法测定 RBC 生存力，绵羊是一种常用的模型。[（14）C]氰酸盐作为长期 RBC 生存力的参考方法，因为（51）Cr 方法（人类的参考方法）在绵羊中不可靠。
从 8 只成年绵羊采集的自体 RBC 等分试样用[（14）C]氰酸盐和 4 种不同密度的生物素（BioRBCs）标记，然后再回输。通过输注后 24 小时的恢复程度（PTR（24））评估短期 RBC 生存力；通过半衰期（T（50））和平均潜在寿命（MPL）评估长期 RBC 生存力。
4 种 BioRBC 密度的 PTR（24）值没有差异。T（50）和 MPL 反映的 RBC 生存力值对于[（14）C]氰酸盐和两种中等密度的 BioRBC 群体几乎相同。相比之下，最低密度的 BioRBC 群体的生存时间略长（p<0.01），但差异无临床意义。最高密度的 BioRBC 群体显著缩短了 RBC 生存力（与两种中等密度相比，p<0.01）。
这项研究提供了证据，表明可以使用标记了 4 种生物素密度的 BioRBCs 独立且同时测量短期 RBC 生存力，并且可以使用标记了 3 种最低生物素密度的 BioRBCs 准确且同时测量长期 RBC 生存力。由于绵羊 RBC 模型与人相似，因此这种非放射性方法有望用于新生儿和孕妇的 RBC 动力学研究。
