Department of Pediatrics, University of Iowa, Iowa City, Iowa.
Pediatr Res. 2013 Nov;74(5):592-600. doi: 10.1038/pr.2013.143. Epub 2013 Sep 3.
Study of the pathophysiology and treatment of anemia of prematurity is facilitated by direct measurement of red cell volume (RCV) utilizing microliter quantities of blood samples. Our objective was to compare concurrent measurements of multiple direct RCV methods in infants.
Eighteen preterm infants receiving clinically indicated transfusions had concurrent flow cytometric determinations of RCV and 24-h red blood cell (RBC) recovery based on donor-recipient differences of biotin-labeled RBCs (BioRBCs), Kidd antigen mismatched RBCs, and fetal hemoglobin-positive (HbF(+)) RBCs. High-performance liquid chromatography (HPLC) was also used for measuring HbF and adult hemoglobin protein concentrations for the determination of RCV.
Concurrent RCV measurements using BioRBCs (18 and 54 µg/ml), Kidd antigen, and HbF flow cytometry were not statistically different compared with RCVs measured using the reference BioRBC density (6 µg/ml). By contrast, the HbF-HPLC method overestimated RCV by 45% compared with the reference method. All the methods demonstrated 100% 24-h posttransfusion RBC recovery (PTR24).
Because BioRBC, Kidd antigen, and fetal hemoglobin (HbF) flow cytometry are safe and accurate methods requiring <10 µl of patient blood for determining RCV and PTR24 in preterm infants, they can be useful in clinical and research studies of anemia and other conditions.
通过直接测量红细胞体积（RCV）利用微升数量的血液样本，可以促进早产儿贫血的病理生理学和治疗的研究。我们的目的是比较在婴儿中同时测量多种直接 RCV 方法。
18 名接受临床指示输血的早产儿同时进行流式细胞术测定 RCV 和基于供体-受者差异的生物素标记红细胞（BioRBC）、Kidd 抗原不相合 RBC 和胎儿血红蛋白阳性（HbF(+)）RBC 的 24 小时 RBC 恢复。高效液相色谱法（HPLC）也用于测量 HbF 和成人血红蛋白蛋白浓度以确定 RCV。
与参考 BioRBC 密度（6 µg/ml）相比，使用 BioRBC（18 和 54 µg/ml）、Kidd 抗原和 HbF 流式细胞术进行的同时 RCV 测量在统计学上没有差异。相比之下，HbF-HPLC 方法比参考方法高估了 45%的 RCV。所有方法均显示 100%的转铁后 24 小时 RBC 恢复（PTR24）。
由于 BioRBC、Kidd 抗原和胎儿血红蛋白（HbF）流式细胞术是安全且准确的方法，仅需<10 µl 患者血液即可确定早产儿的 RCV 和 PTR24，因此它们可用于贫血和其他疾病的临床和研究。
