Leitch R A, Richards J C
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ont.
Biochem Cell Biol. 1990 Apr;68(4):778-89. doi: 10.1139/o90-112.
The specific capsular polysaccharide produced by Rhodococcus equi serotype 1 was found to be a high molecular weight acidic polymer composed of D-glucose, D-mannose, and D-glucuronic acid. Structural analysis of the polysaccharide employed a combination of chemical and nuclear magnetic resonance techniques, from which it was determined that the polysaccharide possessed a linear repeating tetrasaccharide unit containing a single O-acetyl substituent and and acetal-linked pyruvic acid moiety: [formula: see text] The 1H and 13C nuclear magnetic resonances of O-deacetylated and pyruvic-free serotype 1 polysaccharides were fully assigned by homo- and hetero-nuclear chemical shift correlation methods.
已发现马红球菌1型血清型产生的特定荚膜多糖是一种由D-葡萄糖、D-甘露糖和D-葡萄糖醛酸组成的高分子量酸性聚合物。多糖的结构分析采用了化学和核磁共振技术相结合的方法，由此确定该多糖具有一个线性重复四糖单元，其中含有一个单一的O-乙酰基取代基和一个缩醛连接的丙酮酸部分：[分子式：见正文] 通过同核和异核化学位移相关方法，对O-脱乙酰化和无丙酮酸的1型血清型多糖的1H和13C核磁共振进行了完全归属。
