马红球菌7型特异性荚膜多糖的结构解析

Structural elucidation of the specific capsular polysaccharide of Rhodococcus equi serotype 7.

作者信息

Masoud H, Richards J C

机构信息

Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario.

出版信息

Carbohydr Res. 1994 Jan 15;252:223-33. doi: 10.1016/0008-6215(94)90017-5.

DOI:10.1016/0008-6215(94)90017-5

PMID:8137362

Abstract

Structural analysis of the specific capsular polysaccharide produced by Rhodococcus equi serotype 7 indicated it to be a high-molecular-weight polymer consisting of equal molar amounts of D-galactose, D-mannose, L-rhamnose, and pyruvic acid. By employing a combination of chemical and NMR techniques, it was established that the polysaccharide is composed of the linear repeating trisaccharide units: [formula: see text] -->3)-alpha-D-Galp-(1-->3)-alpha-D-Manp-(1-->3)-alpha-L-Rhap -(1-->, in which the cyclic pyruvic acid acetal groups bridging the O-4 and O-6 positions of the alpha-D-Manp residues have the S-configuration. The 1H and 13C NMR spectra of the native and pyruvic acetal-free polysaccharides were fully assigned.

摘要

马红球菌7型产生的特异性荚膜多糖的结构分析表明，它是一种高分子量聚合物，由等摩尔量的D-半乳糖、D-甘露糖、L-鼠李糖和丙酮酸组成。通过结合化学和核磁共振技术，确定该多糖由线性重复三糖单元组成：[化学式：见正文] -->3)-α-D-半乳糖-(1-->3)-α-D-甘露糖-(1-->3)-α-L-鼠李糖-(1--，其中连接α-D-甘露糖残基的O-4和O-6位置的环状丙酮酸缩醛基团具有S-构型。对天然多糖和无丙酮酸缩醛多糖的1H和13C核磁共振谱进行了完全归属。