College of Environmental and Natural Resource Sciences, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou, China.
Environ Pollut. 2012 Feb;161:121-7. doi: 10.1016/j.envpol.2011.09.038. Epub 2011 Nov 4.
Biodegradation processes and changes in microbial community structure were investigated in black carbon (BC) amended soils in a laboratory experiment using two soils (black soil and red soil). We applied different percentages of charcoal as BC (0%, 0.5% and 1% by weight) with 100 mg kg(-1) of phenanthrene. Soil samples were collected at different incubation times (0, 7, 15, 30, 60, 120 d). The amendment with BC caused a marked decrease in the dissipation (ascribed to mainly degradation and/or sequestration) of phenanthrene residues from soil. Extracted phenanthrene in black soil with 1% BC were higher, oppositely in red soil, 0.5% BC amendments were higher. There were significant changes in the PLFA pattern in phenanthrene-spiked soils with time but BC had little effect on the microbial community structure of phenanthrene-spiked soils, as indicated by principal component analysis (PCA) of the PLFA signatures.
采用实验室试验，研究了添加生物炭（BC）对两种土壤（黑土和红土）中微生物群落结构变化和生物降解过程的影响。我们施加了不同比例的生物炭（占土壤重量的 0％、0.5％和 1％），并用 100mgkg(-1)的菲进行处理。在不同的培养时间（0、7、15、30、60、120d）采集土壤样品。BC 的添加导致土壤中菲残留的消解（主要归因于降解和/或固定）显著减少。添加 1％BC 的黑土中提取的菲含量较高，而在红土中，添加 0.5％BC 的土壤中提取的菲含量较高。随着时间的推移，添加菲的土壤中 PLFA 模式发生了显著变化，但主成分分析（PCA）表明，BC 对添加菲的土壤微生物群落结构几乎没有影响。
