Environmental NMR Centre, Department of Physical and Environmental Sciences, University of Toronto, 1265 Military Trail Toronto, Ontario M1C 1A4, Canada.
Environ Pollut. 2013 Apr;175:75-81. doi: 10.1016/j.envpol.2012.12.018. Epub 2013 Jan 19.
We evaluated the correlation between soil organic carbon (OC) content and metabolic responses of Eisenia fetida earthworms after exposure to phenanthrene (58 ± 3 mg/kg) spiked into seven artificial soils with OC contents ranging from 1 to 27% OC. Principal component analysis of (1)H nuclear magnetic resonance (NMR) spectra of aqueous extracts identified statistically significant differences in the metabolic profiles of control and phenanthrene-exposed E. fetida in the 1% OC soil only. Partial least squares analysis identified a metabolic response in the four soils with OC values ≤11% which was well correlated to estimated phenanthrene porewater concentrations. The results suggest that the higher sorption capability of high OC soils decreased the bioavailability of phenanthrene and the subsequent metabolic response of E. fetida.
我们评估了土壤有机碳（OC）含量与接触到菲（58 ± 3mg/kg）的赤子爱胜蚓代谢反应之间的相关性，菲被添加到 OC 含量从 1%到 27%的七种人工土壤中。水提物的（1）H 核磁共振（NMR）图谱的主成分分析仅在 OC 为 1%的土壤中鉴定出对照组和菲暴露组赤子爱胜蚓代谢谱有统计学显著差异。偏最小二乘分析鉴定出在 OC 值≤11%的四种土壤中存在代谢反应，与估计的菲水相浓度很好地相关。结果表明，高 OC 土壤较高的吸附能力降低了菲的生物利用度，以及赤子爱胜蚓的后续代谢反应。
