Seftor E A, Seftor R E, Hendrix M J
University of Arizona.
Biotechniques. 1990 Sep;9(3):324-31.
The formation and propagation of several subpopulations of human melanoma cells from a heterogeneous parental population was accomplished with the use of the Membrane Invasion Culture System (MICS) in vitro under sterile conditions. Five sequentially selected subpopulations of melanoma cells showed an increasing ability to do the following: a) invade reconstituted basement membranes in vitro; b) form experimental lung metastases in vivo; and c) express steady-state levels of human type IV collagenase, a marker for metastatic potential. In addition, the morphology and expression of 35S-methionine-labeled cell surface proteins changed with sequential selection. The adaptation of the MICS assay for studying tumor cell subpopulations allows the morphological, biochemical and molecular characterization of events associated with tumor progression in an in vitro model.
在无菌条件下，利用膜侵袭培养系统（MICS）在体外实现了从异质性亲代群体中形成并传代培养几个人类黑色素瘤细胞亚群。依次选取的五个黑色素瘤细胞亚群在以下方面表现出不断增强的能力：a）在体外侵袭重组基底膜；b）在体内形成实验性肺转移瘤；c）表达人IV型胶原酶的稳态水平，这是转移潜能的一个标志物。此外，随着依次选取，35S-甲硫氨酸标记的细胞表面蛋白的形态和表达发生了变化。MICS检测法适用于研究肿瘤细胞亚群，这使得在体外模型中能够对与肿瘤进展相关的事件进行形态学、生物化学和分子特征分析。
