Kozlowski J M, Hart I R, Fidler I J, Hanna N
J Natl Cancer Inst. 1984 Apr;72(4):913-7.
The ability of the human A375 melanoma cell line to produce experimental and spontaneous in young BALB/c nude mice was examined. Cloned lines, obtained by isolation in semisolid agar, differed significantly (4/10 cloned lines examined, P less than or equal to .005) from the parent tumor line with regard to their ability to form lung tumor nodules subsequent to iv injection. Lines established from such lung tumor nodules were more metastatic than the parent line following both iv and sc injection. These results show that the human melanoma cell line used in these studies contained cells with diverse metastatic potential and suggest that metastasis in the nude mouse results from the preferential selection of metastatic subpopulations.
研究了人类A375黑色素瘤细胞系在年轻BALB/c裸鼠体内产生实验性和自发性转移的能力。通过在半固体琼脂中分离获得的克隆细胞系，在静脉注射后形成肺肿瘤结节的能力方面，与亲代肿瘤细胞系有显著差异（在检测的10个克隆细胞系中有4个，P≤0.005）。从这些肺肿瘤结节建立的细胞系在静脉注射和皮下注射后比亲代细胞系更具转移性。这些结果表明，本研究中使用的人类黑色素瘤细胞系包含具有不同转移潜能的细胞，并提示裸鼠体内的转移是由转移性亚群的优先选择导致的。
