Landymore R W, MacAulay M A, Manku M S
Department of Surgery, Dalhousie University, Halifax, N.S., Canada.
Eur J Cardiothorac Surg. 1990;4(8):441-4. doi: 10.1016/1010-7940(90)90075-b.
Bilateral femoral vein grafts were implanted in 47 adult mongrel dogs to determine the effects of aspirin on intimal hyperplasia. The animals were fed a commercially-prepared 2% cholesterol diet before and for 6 weeks following operation. Twelve animals served as the controls while the remaining animals were divided into three groups receiving low, medium, and high dose aspirin. Eleven animals received 75 mg of aspirin daily, 11 animals were fed 225 mg of aspirin daily and the remaining 13 animals received 650 mg of aspirin daily. A coagulation profile was carried out before operation and at 2, 4 and 6 weeks following operation. The grafts were harvested at 6 weeks and intimal thickness was measured with a Zeiss computerized interactive image-analyzing system. The prothrombin time, partial thromboplastin time, and platelet count were unchanged in all animals. The bleeding time was prolonged in animals receiving aspirin (P less than 0.02). Intimal thickness measured 4 +/- 0.2 microns before implantation and increased at 6 weeks to 39 +/- 5 microns in the control group. Aspirin failed to reduce intimal hyperplasia. Intimal thickness measured 37 +/- 2 microns in those animals receiving 75 mg of aspirin daily, 35 +/- 3 microns after a daily dose of 225 mg of aspirin and 51 +/- 4 microns in the high dose group receiving 650 mg of aspirin daily. Our data indicates that aspirin fails to reduce intimal proliferation in canine vein grafts which suggests that alternative or combined drug therapy may be necessary to reduce the incidence of late graft failure.
将47只成年杂种犬植入双侧股静脉移植物，以确定阿司匹林对内膜增生的影响。在手术前及术后6周，给这些动物喂食市售的2%胆固醇饮食。12只动物作为对照，其余动物分为三组，分别接受低、中、高剂量的阿司匹林。11只动物每天接受75毫克阿司匹林，11只动物每天喂食225毫克阿司匹林，其余13只动物每天接受650毫克阿司匹林。在手术前以及术后2周、4周和6周进行凝血指标检测。在6周时取出移植物，用蔡司计算机交互式图像分析系统测量内膜厚度。所有动物的凝血酶原时间、部分凝血活酶时间和血小板计数均无变化。接受阿司匹林的动物出血时间延长（P<0.02）。植入前内膜厚度为4±0.2微米，对照组在6周时增加到39±5微米。阿司匹林未能减少内膜增生。每天接受75毫克阿司匹林的动物内膜厚度为37±2微米，每天服用225毫克阿司匹林后为35±3微米，每天接受650毫克阿司匹林的高剂量组为51±4微米。我们的数据表明，阿司匹林未能减少犬静脉移植物中的内膜增殖，这表明可能需要替代或联合药物治疗以降低晚期移植物失败的发生率。
