Landymore R W, Kinley C E, Cooper J H, MacAulay M, Sheridan B, Cameron C
J Thorac Cardiovasc Surg. 1985 Mar;89(3):351-7.
Cod-liver oil, rich in eicosapentaenoic acid, an unsaturated fatty acid, was administered to 14 mongrel dogs to determine if this acid would prevent platelet-mediated intimal hyperplasia. Twenty-eight 1 cm segments of undistended jugular vein were interposed between bilaterally divided femoral arteries. Seven control animals were fed a 2% cholesterol diet 1 week before and for 6 weeks after the operation. A further seven animals received cod-liver oil capsules containing 1.8 gm of eicosapentaenoic acid daily 1 week before and for 6 weeks after autogenous vein implantation, in addition to the lipid-supplemented diet. Baseline serum cholesterol was 4.6 +/- 0.4 mmol/L. The rise in serum cholesterol was similar in the two groups and increased to 7.4 +/- 0.6 mmol/L (control group) and to 6.8 +/- 0.2 mmol/L (eicosapentaenoic acid group) (p less than 0.001). Prothrombin time, partial thromboplastin time, bleeding time, and platelet counts were unchanged in the two groups. Vein grafts, harvested at 6 weeks, were fixed in formaldehyde. Mean intimal thickness was measured from multiple vein graft cross sections with a Zeiss computerized interactive image analyzing system. A mean of 140 +/- 11 measurements were computed from each graft. Marked intimal hyperplasia occurred in the control group and increased from 4.3 +/- 0.3 to 86.4 +/- 14 micron. In contrast, a high eicosapentaenoic acid diet inhibited intimal hyperplasia, with intimal thickness only increasing from 4.0 +/- 0.4 to 24.8 +/- 2.7 micron (p less than 0.001). These data indicate that eicosapentaenoic acid inhibits platelet-mediated intimal hyperplasia and suggest that cod-liver oil could be used to prevent intimal hyperplasia in vein grafts used for myocardial revascularization.
将富含不饱和脂肪酸二十碳五烯酸的鱼肝油喂给14只杂种狗，以确定该酸是否能预防血小板介导的内膜增生。将28段未扩张的1厘米颈静脉段置于双侧切断的股动脉之间。7只对照动物在手术前1周和手术后6周喂食2%胆固醇饮食。另外7只动物在自体静脉植入前1周和植入后6周，除了补充脂质的饮食外，每天还接受含有1.8克二十碳五烯酸的鱼肝油胶囊。基线血清胆固醇为4.6±0.4毫摩尔/升。两组血清胆固醇的升高相似，分别升至7.4±0.6毫摩尔/升（对照组）和6.8±0.2毫摩尔/升（二十碳五烯酸组）（p<0.001）。两组的凝血酶原时间、部分凝血活酶时间、出血时间和血小板计数均未改变。6周时采集的静脉移植物用甲醛固定。使用蔡司计算机交互式图像分析系统从多个静脉移植物横截面测量平均内膜厚度。每个移植物计算出平均140±11次测量值。对照组出现明显的内膜增生，从4.3±0.3微米增加到86.4±14微米。相比之下，高二十碳五烯酸饮食可抑制内膜增生，内膜厚度仅从4.0±0.4微米增加到24.8±2.7微米（p<0.001）。这些数据表明，二十碳五烯酸可抑制血小板介导的内膜增生，并提示鱼肝油可用于预防用于心肌血运重建的静脉移植物中的内膜增生。
