Parish R W
Biochim Biophys Acta. 1979 May 3;553(1):179-82. doi: 10.1016/0005-2736(79)90040-3.
Dictyostelium discoideum slugs (pseudoplasmodia) were disaggregated and the resynthesis of developmentally regulated plasma membrane proteins examined. The synthesis of the majority of these proteins was inhibited when cells were overlaid with Cellophane and maintained as a monolayer. However, cell contact and movement did occur under the Cellophane. The inhibition of differentiation may result from the inability of the cells to organize specifically into multicellular aggregates. The addition of cyclic AMP (1--5 mM) induced the synthesis of certain developmentally regulated plasma membrane proteins in cells overlaid with Cellophane. Hence, this confirms other work showing that cyclic AMP is required for at least some post-aggregative gene expression. Specific cell organisation and interactions are apparently required for an increase in or maintenance of intracellular cyclic AMP levels.
将盘基网柄菌的蛞蝓体（假原质团）分散开，并检测发育调控的质膜蛋白的重新合成。当用玻璃纸覆盖细胞并使其保持单层状态时，这些蛋白中的大多数的合成受到抑制。然而，在玻璃纸下细胞确实发生了接触和移动。分化的抑制可能是由于细胞无法特异性地组织成多细胞聚集体。添加环磷酸腺苷（1 - 5 mM）可诱导用玻璃纸覆盖的细胞中某些发育调控的质膜蛋白的合成。因此，这证实了其他研究结果，即环磷酸腺苷对于至少一些聚集后基因表达是必需的。细胞内的环磷酸腺苷水平的升高或维持显然需要特定的细胞组织和相互作用。
