Sakono Masafumi, Zako Tamotsu, Maeda Mizuo
PRESTO, Japan Science and Technology Agency, Sanbancho, Chiyoda, Tokyo, Japan.
Anal Sci. 2012;28(1):73. doi: 10.2116/analsci.28.73.
We developed a rapid method for estimating the amyloid beta (Aβ)-conformation state related with Alzheimer's disease. We prepared gold nanoparticle (AuNP)-Aβ antibody conjugates treated with bovine serum albumin to stabilize their dispersibility in a buffer. The prepared AuNPs were precipitated in the presence of Aβ aggregates, such as oligomers and fibrils. Aβ monomers did not precipitate AuNPs. The formation of AuNP precipitates by Aβ aggregates could be confirmed by the naked eye within 1 h.
我们开发了一种快速方法来估计与阿尔茨海默病相关的β-淀粉样蛋白（Aβ）构象状态。我们制备了用牛血清白蛋白处理的金纳米颗粒（AuNP）-Aβ抗体缀合物，以稳定它们在缓冲液中的分散性。所制备的金纳米颗粒在Aβ聚集体（如寡聚体和原纤维）存在的情况下会沉淀。Aβ单体不会使金纳米颗粒沉淀。Aβ聚集体导致的金纳米颗粒沉淀的形成在1小时内可用肉眼确认。
