有袋类动物和单孔目动物的基因图谱。II. 袋鼬科有袋类动物X染色体的基因定位

Gene mapping in marsupials and monotremes. II. Assignments to the X chromosome of dasyurid marsupials.

作者信息

Dobrovic A, Marshall Graves J A

机构信息

Department of Genetics, La Trobe University, Melbourne, Australia.

出版信息

Cytogenet Cell Genet. 1986;41(1):9-13. doi: 10.1159/000132189.

DOI:10.1159/000132189

PMID:22232817

Abstract

Somatic cell hybrids have been obtained between HPRT Chinese hamster cells and cells from several dasyurid marsupial species. These hybrids show the extensive loss of marsupial chromosomes characteristic of the majority of marsupial-eutherian somatic cell hybrids. Although all of the hybrids expressed the selected marsupial marker, HPRT, the only other markers observed were PGK, GLA, and G6PD, consistent with the conservation of X-linked genes extending to this major group of marsupials. Counterselection confirmed the synteny of PGK and GLA with HPRT, whereas G6PD showed decreased concordance.

摘要

已获得中国仓鼠次黄嘌呤磷酸核糖转移酶（HPRT）细胞与几种袋鼬科有袋动物细胞之间的体细胞杂种。这些杂种显示出大多数有袋类-真兽类体细胞杂种所特有的有袋动物染色体的大量丢失。尽管所有杂种都表达了选定的有袋动物标记HPRT，但观察到的唯一其他标记是磷酸甘油酸激酶（PGK）、α-半乳糖苷酶A（GLA）和葡萄糖-6-磷酸脱氢酶（G6PD），这与X连锁基因的保守性延伸到这一主要有袋动物群体是一致的。反选择证实了PGK和GLA与HPRT的同线性，而G6PD显示出较低的一致性。