含有有袋类动物X染色体片段的有袋类动物-小鼠细胞杂种。

Marsupial--mouse cell hybrids containing fragments of the marsupial X chromosome.

作者信息

Graves J A, Chew G K, Cooper D W, Johnston P G

出版信息

Somatic Cell Genet. 1979 Jul;5(4):481-9. doi: 10.1007/BF01538882.

Abstract

Hybrids were obtained from fusions of HPRT-deficient mouse fibroblasts and marsupial lymphocytes. These hybrids retained no identifiable marsupial chromosomes, but all expressed the marsupial form of HPRT. Half the clones also expressed marsupial PGK-A, and half of these also marsupial G6PD; no other marsupial allozyme markers were detected. Since G6PD is known to be sex linked in these species, we conclude that Hpt and Pgk-A are also located on the X chromosome and the markers lie in the order Hpt-Pgk-A-Gpd.

摘要

通过将缺乏次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）的小鼠成纤维细胞与有袋动物淋巴细胞融合获得了杂种细胞。这些杂种细胞没有保留可识别的有袋动物染色体，但都表达了有袋动物形式的HPRT。一半的克隆还表达了有袋动物的磷酸甘油酸激酶-A（PGK-A），其中一半还表达了有袋动物的葡萄糖-6-磷酸脱氢酶（G6PD）；未检测到其他有袋动物同工酶标记。由于已知G6PD在这些物种中是性连锁的，我们得出结论，Hpt和Pgk-A也位于X染色体上，并且这些标记的排列顺序为Hpt-Pgk-A-Gpd。