有袋类动物和单孔目动物的基因图谱，V. 鸭嘴兽中次黄嘌呤磷酸核糖基转移酶与磷酸甘油酸激酶之间的同线性
Gene mapping in marsupials and monotremes, V. Synteny between hypoxanthine phosphoribosyltransferase and phosphoglycerate kinase in the platypus.
作者信息
Watson J M, Graves J A
机构信息
Department of Genetics and Human Variation, La Trobe University, Bundoora.
出版信息
Aust J Biol Sci. 1988;41(2):231-7. doi: 10.1071/bi9880231.
In order to extend comparative mapping studies to the monotreme mammals (subclass Prototheria), somatic-cell hybrids were obtained between Chinese-hamster cells deficient in hypoxanthine phosphoribosyltransferase (HPRT) and platypus fibroblasts. The characteristics of these hybrids closely resemble those of metatherian x eutherian hybrids, in that they are recovered at low frequency and they rapidly segregate and fragment platypus chromosomes. Biochemical and cytological studies of the hybrids, their subclones and HPRT-deficient revertants indicate that phosphoglycerate kinase is syntenic with HPRT in the platypus (as it is in other mammals); however, the studies do not permit chromosomal assignment of the syntenic group. The implications of the chromosomal location of this ancient synteny group for the evolution of the mammalian X chromosome are discussed.
为了将比较图谱研究扩展到单孔目哺乳动物（原兽亚纲），在缺乏次黄嘌呤磷酸核糖基转移酶（HPRT）的中国仓鼠细胞与鸭嘴兽成纤维细胞之间获得了体细胞杂种。这些杂种的特征与有袋类与真兽类杂种的特征非常相似，即它们的获得频率很低，并且它们会迅速分离并使鸭嘴兽染色体断裂。对杂种、它们的亚克隆以及HPRT缺陷回复体的生化和细胞学研究表明，磷酸甘油酸激酶在鸭嘴兽中与HPRT同线（就像在其他哺乳动物中一样）；然而，这些研究无法确定该同线群的染色体定位。讨论了这个古老同线群的染色体位置对哺乳动物X染色体进化的影响。
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