Agadzhanian A A, Asaturian R A, Ambartsumian A A, Sargisian L B, Ovsepian A S, Vardanian A H, Sagiian A S
Prikl Biokhim Mikrobiol. 2011 Sep-Oct;47(5):551-7.
The effective sorption method for melanin isolation and purification from fermentation solutions of Bacillus thuringiensis serovar galleriae K1 has been elaborated, the principle process flowsheet is presented. The identification of obtained pigment with the samples of natural and synthetic melanin was done by IR-spectroscopy, and the intensity ratio of optical absorption at 650 and 500 nm allows to refer the isolated melanin to eumelanin class. By thermal treatment it was determined, that melanin's amorphous sediment is steady at up to 120 degrees C temperatures, at that the concentration of paramagnetic centers is changed from 0.053 x 10(18) spin/g (48 degrees C) to 0.25 x 10(18) spin/g (120 degrees C). The rising of the temperature of treatment up to 210 degrees C brings to substantial increase of the concentration of unpaired electrons, and at 280 degrees C its sharp growth is observed. At 350 degrees C growth stops, then sharp decrease is observed. The obtained results were confirmed by methods of IR spectroscopy and derivatographic analysis.
已阐述了从苏云金芽孢杆菌蜡螟亚种K1发酵液中分离和纯化黑色素的有效吸附方法，并给出了主要工艺流程。通过红外光谱对获得的色素与天然和合成黑色素样品进行了鉴定，650和500nm处光吸收的强度比表明分离出的黑色素属于真黑色素类别。通过热处理确定，黑色素的无定形沉淀物在高达120℃的温度下是稳定的，此时顺磁中心的浓度从0.053×10(18)自旋/克（48℃）变为0.25×10(18)自旋/克（120℃）。处理温度升至210℃会导致未成对电子浓度大幅增加，在280℃时观察到其急剧增长。在350℃时增长停止，随后观察到急剧下降。所得结果通过红外光谱法和衍生色谱分析方法得到了证实。
