Hoti S L, Balaraman K
Vector Control Research Centre, ICMR, Indira Nagar, Pondicherry, India.
J Gen Microbiol. 1993 Oct;139(10):2365-9. doi: 10.1099/00221287-139-10-2365.
A mutant of Bacillus thuringiensis H-14 produced a dark brown pigment during sporulation. Production of the pigment depended on the nutritional properties of the growth medium. The pigment was identified as melanin, based on chemical tests and its infra-red spectrum. Incorporation of L-tyrosine in the culture medium enhanced the level of melanin production, and L-3,4-dihydroxyphenylalanine (L-DOPA) was detected in the culture broth during the late-exponential phase of growth. This indicates that the pathway of melanin synthesis is from L-tyrosine, via L-DOPA, to melanin.
苏云金芽孢杆菌H-14的一个突变体在芽孢形成过程中产生了深棕色色素。色素的产生取决于生长培养基的营养特性。基于化学测试及其红外光谱，该色素被鉴定为黑色素。在培养基中添加L-酪氨酸可提高黑色素的产生水平，并且在生长的指数后期阶段在培养液中检测到了L-3,4-二羟基苯丙氨酸（L-DOPA）。这表明黑色素的合成途径是从L-酪氨酸经L-DOPA到黑色素。
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