Rukavishnikova G E, Alekseeva A K
Acta Virol. 1977 Sep;21(5):391-6.
Influenza A virus antigens were detected in leukocytes by immunofluorescence. After intravenous inoculation of the A/Moscow/16/65 (H2N2) vaccine strain to chickens, cytoplasmic antigens of the virus were observed in mononuclear leukocytes from 24 to 72 hours post inoculation (p.i.). The course of antigen detectability was similar after two repeated inoculations of the virus. After oral vaccination of human volunteers with a live tissue culture influenza A monovaccine from the X-47 (H3N2) recombinant viral antigens were also found in mononuclears; the maximal number of antigen-positive cells was observed at 24 hours p.i. The method of membrane immunofluorescence proved to be the most sensitive for antigen detection; it revealed a considerable decrease in the number of antigen-positive cells after repeated administration of the virus to volunteers. This fact may possibly reflect the development of antiviral resistance in the process of vaccination.
通过免疫荧光法在白细胞中检测到甲型流感病毒抗原。给鸡静脉接种A/莫斯科/16/65（H2N2）疫苗株后，接种后24至72小时在单核白细胞中观察到病毒的细胞质抗原。两次重复接种病毒后，抗原可检测性过程相似。用来自X - 47（H3N2）的活组织培养甲型流感单价疫苗口服接种人类志愿者后，在单核细胞中也发现了重组病毒抗原；在接种后24小时观察到抗原阳性细胞的最大数量。膜免疫荧光法被证明是检测抗原最敏感的方法；在向志愿者重复给药病毒后，它显示抗原阳性细胞数量显著减少。这一事实可能反映了疫苗接种过程中抗病毒抗性的发展。
