[通过引入超声处理优化桦褐孔菌免疫活性多糖的提取工艺]
[Process optimization for extraction of immune active polysaccharides from Fomes fomentarius by introduction of ultrasonication].
作者信息
He Gai-Yi-Nan, Lei Lin-Sheng, Chen Jie, Yu Chuan-Lin, Ren Jing, Chen Na-Na
机构信息
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
出版信息
Zhong Yao Cai. 2011 Aug;34(8):1277-80.
OBJECTIVE
To study the optimal extraction process of immune active polysaccharides from Fomes fomentarius by introduction of ultrasonication.
METHODS
An orthogonal experimental design of L9 (3(4)) was used to investigate the effects of ultrasonication time, extraction temperature and extraction time on the extraction ratio, sugar content and immune stimulating activity (mouse splenocyte metabolic activity measured with MTT colorimetry) of the polysaccharides and the optimal extraction process was evaluated.
RESULTS
Ultrasonication treatment had the most remarkable effect on the immune stimulating activity of the polysaccharides. The optimal extraction process for extraction of immune active polysaccharides was as follows: ultrasonication for 30min, extraction temperature at 80 degrees C and water extraction time for 2h.
CONCLUSION
Ultrasonication can be used as a useful technique for extraction of immune active polysaccharides from Fomes fomentarius.
目的
通过引入超声处理研究从桦褐孔菌中提取免疫活性多糖的最佳工艺。
方法
采用L9(3(4))正交试验设计，考察超声时间、提取温度和提取时间对多糖提取率、糖含量及免疫刺激活性（用MTT比色法测定小鼠脾细胞代谢活性）的影响，并评价最佳提取工艺。
结果
超声处理对多糖的免疫刺激活性影响最为显著。提取免疫活性多糖的最佳工艺如下：超声处理30分钟，提取温度80℃，水提取时间2小时。
结论
超声处理可作为从桦褐孔菌中提取免疫活性多糖的有效技术。
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