[正交试验设计优化太白米党参多糖提取工艺]
[Optimization for extraction technology of polysaccharide in root of Adenophora potaninii from Taibai mountain in China by orthogonal experimental design].
作者信息
Li Peng-Cheng, Zhang Ben-Cai, Chen Zhan-Guo
机构信息
College of Chemistry and Material Science, Shanxi Normal University, Xi'an 710062, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Jun;33(11):1266-8, 1312.
OBJECTIVE
This study is to develop excellent extraction technology of the polysaccharides in the root of the A. potaninii which live in the Taibai Mountain in China.
METHOD
Based on the extraction with water, the polysaccharides were deposited with alcohol. With the content of polysaccharides was as the index, extraction conditions were investigated systemly. Employed the solid-liquid ratio, extraction temperature, extraction time and extraction number of times were as levels of single factor, the optimal extraction technology of the polysaccharides from the root of the A. potaninii was determined by L9 (3(4)) orthogonal experimental design L9 (3(4)).
RESULT
The optimal technology conditions were that the solid-liquid ratio was 1 : 30, the extracting temperature was 60 degrees C, the extracting time was 3 h and extracting number of times was 3 times.
CONCLUSION
The optimized extraction technology is simple, reliable and extraction efficiency of polysaccharide is higher.
目的
本研究旨在开发中国太白山产太白党参根中多糖的优良提取工艺。
方法
以水提取，乙醇沉淀多糖。以多糖含量为指标，系统考察提取条件。采用料液比、提取温度、提取时间和提取次数作为单因素水平，通过L9(3(4))正交试验设计确定太白党参根中多糖的最佳提取工艺。
结果
最佳工艺条件为料液比1∶30、提取温度60℃、提取时间3 h、提取次数3次。
结论
优化后的提取工艺简单、可靠，多糖提取效率较高。
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