Ozyilmaz Ezgi, Yildirim Beytullah, Aydogdu Muge, Dincel Aylin Sepici, Elmas Cigdem, Oguzulgen I Kivilcim, Tuncer Candan
Department of Pulmonary Disease, Gazi University Faculty of Medicine, Ankara, Turkey.
Hepatogastroenterology. 2011 Nov-Dec;58(112):1898-903. doi: 10.5754/hge10094.
BACKGROUND/AIMS: Lung involvement due to inflammatory bowel disease (IBD) is frequent, however the pathogenic mechanism is still debatable. Although the evidence of inflammation in colonic and lung tissue has been documented, the possible effect of oxidative stress in lung tissue has not been evaluated to date. We sought to assess the effects of oxidant/antioxidants on lung tissue in a model of experimental colitis.
Colitis was induced with intra-colonic administration of 4% acetic acid. Control group received isotonic saline. Serum and lung tissue markers of oxidative stress were explored.
Serum total oxidant status was significantly higher in the colitis group than the controls while total antioxidant status was similar. The determinants of oxidants including lipid peroxidation assay and myeloperoxidase activity were significantly higher in the lung tissue of the colitis group whereas the indicators of antioxidant capacity determined as superoxide dismutase, catalase, glutathione and glutathione peroxidase were decreased (p<0.05).
This study showed that oxidative stress is not restricted to the bowel and the lung is a main target of oxidant overload. Pulmonary injury caused by increased oxidant stress may be the underlying reason of pulmonary involvement due to IBD.
背景/目的：炎症性肠病（IBD）导致的肺部受累较为常见，但其致病机制仍存在争议。尽管结肠和肺组织中的炎症证据已有记载，但氧化应激对肺组织可能产生的影响迄今尚未得到评估。我们试图在实验性结肠炎模型中评估氧化剂/抗氧化剂对肺组织的影响。
通过结肠内注射4%乙酸诱导结肠炎。对照组接受等渗盐水。检测血清和肺组织氧化应激标志物。
结肠炎组血清总氧化剂状态显著高于对照组，而总抗氧化剂状态相似。结肠炎组肺组织中包括脂质过氧化测定和髓过氧化物酶活性在内的氧化剂决定因素显著升高，而作为超氧化物歧化酶、过氧化氢酶、谷胱甘肽和谷胱甘肽过氧化物酶测定的抗氧化能力指标则降低（p<0.05）。
本研究表明氧化应激不仅局限于肠道，肺是氧化剂过载的主要靶器官。氧化应激增加导致的肺损伤可能是IBD引起肺部受累的潜在原因。
