Cheglakov I B, Radko S P, Yarygin K N, Vishniakova Kh S, Egorov E E
V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2011 Apr;150(6):744-6. doi: 10.1007/s10517-011-1239-6.
The expression of human telomerase catalytic subunit in HL-60 and HT-1080 malignant transformed cells and telomerized fibroblasts was studied by quantitative PCR. It was found that the number of transcripts of human telomerase catalytic subunit per cell in telomerized fibroblasts could be hundreds of times higher than in HL-60 and HT-1080 cells. Telomerized fibroblast cultures are suggested as experimental systems for selection of basal compounds for creation of anticancer drug prototypes, the molecular target of which is human telomerase catalytic subunit. The effects of human telomerase catalytic subunit expression on the fibroblast proteome are analyzed.
采用定量PCR技术研究了人端粒酶催化亚基在HL-60和HT-1080恶性转化细胞以及端粒化成纤维细胞中的表达情况。结果发现，端粒化成纤维细胞中每个细胞的人端粒酶催化亚基转录本数量可能比HL-60和HT-1080细胞高出数百倍。端粒化成纤维细胞培养物被建议作为筛选抗癌药物原型基础化合物的实验系统，其分子靶点是人端粒酶催化亚基。分析了人端粒酶催化亚基表达对成纤维细胞蛋白质组的影响。
