Khramtsov A V, Ottesen B V
Biokhimiia. 1979 May;44(5):781-8.
The distribution of carbonic anhydrase, K+-ATPase and K+-phosphatase in the subcellular fractions of gastric mucosa was studied. It was found that 90% of carbonic anhydrase are localized in the hyaloplasm, whereas K+-ATPase and K+-phosphatase are predominantly localized in the microsomal fraction. Subfractionation of the microsomal fraction in a sucrose density gradient showed that the membrane-bound carbonic anhydrase (5% of total content) and K+-ATPase are bound to various cell organelles. It is concluded that carbonic anhydrase functions as an intracellular pH-stat and is not directly involved in proton generation by the cell.
研究了碳酸酐酶、钾离子 - 三磷酸腺苷酶（K⁺ - ATPase）和钾离子 - 磷酸酶在胃黏膜亚细胞组分中的分布。发现90%的碳酸酐酶定位于透明质中，而K⁺ - ATPase和K⁺ - 磷酸酶主要定位于微粒体组分中。在蔗糖密度梯度中对微粒体组分进行亚分级分离表明，膜结合的碳酸酐酶（占总含量的5%）和K⁺ - ATPase与各种细胞器结合。得出的结论是，碳酸酐酶作为细胞内pH调节剂发挥作用，并不直接参与细胞产生质子的过程。
