一种人类前列腺癌细胞系对转化生长因子样分子的产生与反应
Production and response of a human prostatic cancer line to transforming growth factor-like molecules.
作者信息
MacDonald A, Chisholm G D, Habib F K
机构信息
University Department of Surgery (WGH), Western General Hospital, Edinburgh, UK.
出版信息
Br J Cancer. 1990 Oct;62(4):579-84. doi: 10.1038/bjc.1990.333.
Abstract
Serum-free media conditioned by the androgen insensitive human prostate cancer cell line DU145 showed immunological transforming growth factor-alpha (TGF alpha) activity, as well as competing activity in epidermal growth factor (EGF) radioreceptor assays (RRA). Furthermore, there were factors in the conditioned media which inhibited and stimulated DNA synthesis by DU145 cells in a dose-dependent fashion. Fractionation of the concentrated conditioned media by reverse-phase high performance liquid chromatography revealed several peaks containing EGF-like competitive activity only one of which demonstrated TGF alpha activity. However, none of the peaks corresponded to immunoreactive EGF. Measurement of EGF receptors on DU145 cells by competition and saturation analysis revealed high levels of receptors (mean +/- s.d. = 2.5 +/- 1 x 10(5) surface receptors per cell) which were of high affinity (Kd +/- s.d. = 1.0 +/- 0.5 nmol l-1). Although DU145 cells express high levels of EGF receptors, DNA synthesis was only minimally affected by exogenous EGF and TGF alpha.
摘要
由雄激素不敏感的人前列腺癌细胞系DU145制备的无血清培养基显示出免疫转化生长因子-α（TGFα）活性，以及在表皮生长因子（EGF）放射受体分析（RRA）中的竞争活性。此外，条件培养基中存在一些因子，它们以剂量依赖的方式抑制和刺激DU145细胞的DNA合成。通过反相高效液相色谱对浓缩的条件培养基进行分级分离，发现几个含有EGF样竞争活性的峰，其中只有一个显示出TGFα活性。然而，没有一个峰对应于免疫反应性EGF。通过竞争和饱和分析测量DU145细胞上的EGF受体，发现受体水平很高（平均±标准差=每个细胞2.5±1×10⁵个表面受体），且具有高亲和力（解离常数±标准差=1.0±0.5 nmol l⁻¹）。尽管DU145细胞表达高水平的EGF受体，但DNA合成仅受到外源性EGF和TGFα的最小影响。
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