Yang Y, Chisholm G D, Habib F K
University Department of Surgery (WGH), Western General Hospital, Edinburgh, Scotland, UK.
Br J Cancer. 1993 Jan;67(1):152-5. doi: 10.1038/bjc.1993.26.
We measured immunoreactive EGF and TGF alpha in prostate tissue extracts obtained from 19 patients with benign prostatic hyperplasia (BPH) and 19 with cancer of the prostate (CaP). Whilst both BPH and CaP expressed EGF (BPH = 195.61 +/- 19.94 ng g-1 protein; CaP = 235.60 +/- 24.45 ng g-1 protein) and TGF alpha (BPH = 92.57 +/- 7.60 ng g-1 protein; CaP = 100.73 +/- 15.47 ng g-1 protein) in equal concentrations, the levels of EGF in any tissue extract were on average twice those of TGF alpha. Furthermore analysis of the individual growth factor data revealed a direct correlation between EGF and TGF alpha in both BPH (r = 0.72, P < 0.001) and CaP (r = 0.69, P < 0.001). When the tumours were classified according to their Gleason score, a slight but significant increase in growth factor concentrations was noted as the tumour became less differentiated. We also measured the concentrations of testosterone and dihydrotestosterone (DHT) in prostate extracts with a view of elucidating the relationship between androgen and growth factors in this gland. There was a small positive correlation only between testosterone and EGF (r = 0.62, P < 0.05) and testosterone and TGF alpha (r = 0.61, P < 0.05) in CaP. The absence of any similar correlation in BPH where DHT becomes the predominant hormone may suggest an indirect role for testosterone in the regulation of growth factor production.
我们检测了19例良性前列腺增生（BPH）患者和19例前列腺癌（CaP）患者前列腺组织提取物中的免疫反应性表皮生长因子（EGF）和转化生长因子α（TGFα）。虽然BPH和CaP均表达EGF（BPH = 195.61±19.94 ng g-1蛋白质；CaP = 235.60±24.45 ng g-1蛋白质）和TGFα（BPH = 92.57±7.60 ng g-1蛋白质；CaP = 100.73±15.47 ng g-1蛋白质），且浓度相等，但任何组织提取物中EGF的水平平均是TGFα的两倍。此外，对个体生长因子数据的分析显示，BPH（r = 0.72，P < 0.001）和CaP（r = 0.69，P < 0.001）中EGF和TGFα之间均存在直接相关性。当根据Gleason评分对肿瘤进行分类时，随着肿瘤分化程度降低，生长因子浓度有轻微但显著增加。我们还检测了前列腺提取物中睾酮和双氢睾酮（DHT）的浓度，以阐明该腺体中雄激素与生长因子之间的关系。在CaP中，仅睾酮与EGF（r = 0.62，P < 0.05）以及睾酮与TGFα（r = 0.61，P < 0.05）之间存在较小的正相关性。在DHT成为主要激素的BPH中不存在任何类似相关性，这可能表明睾酮在生长因子产生的调节中起间接作用。
