Departamento de Bioquímica, Genética e Inmunología, Universidad de Vigo, Vigo, Spain.
Cancer Invest. 2012 Jan;30(1):72-8. doi: 10.3109/07357907.2011.630051.
We studied the specific changes of the secreted protein clusterin and its cytoplasmic precursor regarding colorectal tumorigenesis, using in vitro differentiation of Caco-2 cells. In tumor-like stage, we observed an overexpression of both precursor and secreted clusterin, corroborated in the cell line SW-480. Noticeably, SW-620 cells (from a tumoral node, thus with metastatic capacity) did not show overexpression of either precursor or secreted clusterin, suggesting a downregulation related to local metastasis. We further investigated clusterin in serum, finding a significant increase in colorectal cancer patients, with 81% sensitivity, 79% specificity, and an area under the ROC curve of 0.85.
我们使用 Caco-2 细胞的体外分化研究了分泌蛋白簇蛋白及其细胞质前体在结直肠肿瘤发生过程中的特定变化。在肿瘤样阶段，我们观察到前体和分泌簇蛋白的过度表达，在细胞系 SW-480 中得到证实。值得注意的是，SW-620 细胞（来自肿瘤转移灶，因此具有转移能力）并未显示前体或分泌簇蛋白的过度表达，提示与局部转移相关的下调。我们进一步研究了血清中的簇蛋白，发现结直肠癌患者的血清簇蛋白显著增加，其敏感性为 81%，特异性为 79%，ROC 曲线下面积为 0.85。
