Morgan G J, Hernandez A, Chan L C, Hughes T, Martiat P, Wiedemann L M
Leukaemia Research Fund Centre, Institute of Cancer Research, London.
Br J Haematol. 1990 Sep;76(1):33-8. doi: 10.1111/j.1365-2141.1990.tb07833.x.
Three major types of mRNA can be expressed as a result of the Philadelphia translocation, dependent on the position of the break within the BCR gene on chromosome 22. In addition, alternative splicing of the mRNA transcribed from the BCR/ABL fusion gene has been reported and it has been suggested that this may play a role in the generation of the acute phase of Philadelphia positive chronic myeloid leukaemia (CML). We have examined the fusion RNA present in 24 cases of chronic phase CML and 21 cases of patients with CML in blast crisis using the polymerase chain reaction. In no case was it possible to detect the presence of the e1a2 junction which encodes the p190 hybrid protein product. We conclude that the acquisition of the p190 does not play a significant role in the generation of the blast crisis of CML. Neither could we detect a significant difference in the number of cases which simultaneously express both b2a2 and b3a2 junction products in samples isolated from chronic phase and blast crisis. In the series analysed by ethidium bromide stained gels, there was, however, an increase in the percentage of cases expressing the b3a2 junction in the mononuclear cells of blast crisis patients as compared to the white blood cells of patients in chronic phase.
由于费城染色体易位，根据22号染色体上BCR基因内断裂点的位置，可表达出三种主要类型的mRNA。此外，已有报道称BCR/ABL融合基因转录的mRNA存在可变剪接，有人认为这可能在费城染色体阳性慢性髓性白血病（CML）急性期的发生中起作用。我们使用聚合酶链反应检测了24例慢性期CML患者和21例急变期CML患者中的融合RNA。在任何情况下都无法检测到编码p190杂交蛋白产物的e1a2连接的存在。我们得出结论，p190的获得在CML急变期的发生中不起重要作用。我们也未检测到在慢性期和急变期分离的样本中同时表达b2a2和b3a2连接产物的病例数有显著差异。然而，在通过溴化乙锭染色凝胶分析的系列中，与慢性期患者的白细胞相比，急变期患者单核细胞中表达b3a2连接的病例百分比有所增加。
