Tanaka M, Yamazaki Y, Hattori M, Tsushita K, Utsumi M, Yoshida S
Department of Hematology, Nagoya National Hospital, Japan.
Leuk Res. 1996 Jul;20(7):575-80. doi: 10.1016/0145-2126(96)00010-0.
The hypothesis that minor bcr/abl fusion mRNA is produced in blast crisis of chronic myelogenous leukemia (CML) is examined. The RNA transcripts encoding the minor and major bcr/abl fused protein were detected by polymerase chain reaction (PCR) using RNA from peripheral blood or bone marrow cells of eight patients with blast crisis or accelerated phase of CML. The mRNA encoding for major bcr/abl was detected in all eight cases. In four patients, however, transcripts encoding for minor bcr/abl mRNA were detected, as well as major bcr/abl mRNA. The presence of minor bcr/abl mRNA was verified with the hybridization with a junction-specific probe and DNA sequencing analysis of PCR products. The appearance of minor bcr/abl fusion mRNA was associated with the lymphoblastic immunophenotype of the blast cells. In two of these four patients, samples of initial diagnosis of chronic phase of CML were available, which did not show minor bcr/abl transcript. We conclude that the appearance of minor bcr/abl mRNA transcript is associated with the terminal evolution of CML in lymphoblastic crisis.
对慢性粒细胞白血病（CML）急变期产生微小bcr/abl融合mRNA的假说进行了检验。使用来自8例CML急变期或加速期患者外周血或骨髓细胞的RNA，通过聚合酶链反应（PCR）检测编码微小和主要bcr/abl融合蛋白的RNA转录本。在所有8例病例中均检测到编码主要bcr/abl的mRNA。然而，在4例患者中，除了检测到主要bcr/abl mRNA外，还检测到了编码微小bcr/abl mRNA的转录本。通过与连接特异性探针杂交以及对PCR产物进行DNA测序分析，证实了微小bcr/abl mRNA的存在。微小bcr/abl融合mRNA的出现与原始细胞的淋巴细胞免疫表型相关。在这4例患者中的2例中，有CML慢性期初始诊断的样本，这些样本未显示微小bcr/abl转录本。我们得出结论，微小bcr/abl mRNA转录本的出现与CML淋巴细胞急变期的终末演变相关。
