Department of Orthopaedics, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan, PR China.
Clin Biochem. 2012 Mar;45(4-5):303-8. doi: 10.1016/j.clinbiochem.2011.12.018. Epub 2012 Jan 4.
To screen serum biomarker of knee osteoarthritis (OA) using a phage random peptide library.
A phage random peptide library of random peptide 12-mers was screened with purified immunoglobulin G (IgG) from sera of knee OA patients. Patients with knee rheumatoid arthritis (RA), hip OA, non-erosive hand OA or erosive hand OA, and healthy volunteers were used as controls.
A phage clone with inserted peptide TGLESGHGPGDS (named KOA1) showed 90% positive reaction rate with the knee OA patients, significantly higher than that with the knee RA patients (27.8%), the non-erosive hand OA patients (34.3%), the erosive hand OA patients (31.3%) and the healthy controls (12.0%), but not the hip OA patients (82.5%).
The novel knee OA mimic peptide KOA1 identified with a random phage display peptide library and sera from knee OA patients could be a potential serum biomarker for knee OA.
使用噬菌体随机肽文库筛选膝关节骨关节炎（OA）的血清生物标志物。
用膝关节 OA 患者血清中的纯化免疫球蛋白 G（IgG）筛选噬菌体随机肽 12 肽文库。将膝关节类风湿关节炎（RA）患者、髋关节 OA 患者、非侵蚀性手部 OA 患者、侵蚀性手部 OA 患者和健康志愿者作为对照。
噬菌体克隆插入肽 TGLESGHGPGDS（命名为 KOA1）与膝关节 OA 患者的阳性反应率为 90%，明显高于膝关节 RA 患者（27.8%）、非侵蚀性手部 OA 患者（34.3%）、侵蚀性手部 OA 患者（31.3%）和健康对照组（12.0%），但与髋关节 OA 患者（82.5%）无差异。
用噬菌体随机肽文库和膝关节 OA 患者血清鉴定的新型膝关节 OA 模拟肽 KOA1 可能是膝关节 OA 的潜在血清生物标志物。
