National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
J Hazard Mater. 2012 Feb 29;205-206:150-5. doi: 10.1016/j.jhazmat.2011.12.052. Epub 2011 Dec 26.
Removal of 2,4-dichlorophenol (2,4-DCP) by TiO2/UV photocatalytic, laccase, and simultaneous photocatalytic-enzymatic treatments were investigated. Coupling of native laccase with TiO2/UV showed a negative synergetic effect due to the rapid inactivation of laccase. Immobilizing laccase covalently to controlled porous glass (CPG) effectively enhanced the stability of laccase against TiO2/UV induced inactivation. By coupling CPG-laccase with the TiO2/UV the degradation efficiency of 2,4-DCP was significantly increased as compared with the results obtained when immobilized laccase or TiO2/UV were separately used. Moreover, the enhancement was more remarkable for the degradation of 2,4-DCP with high concentration, such that for the degradation of 5mM 2,4-DCP, 90% removal percentage was achieved within 2h with the coupled degradation process. While for the TiO2/UV and CPG-laccase process, the removal percentage of 2,4-DCP at 2h were only 26.5% and 78.1%, respectively. The degradation kinetics were analyzed using a intermediate model by taking into account of the intermediates formed during the degradation of 2,4-DCP. The high efficiency of the coupled degradation process therefore provided a novel strategy for degradation of concentrated 2,4-DCP. Furthermore, a thermometric biosensor using the immobilized laccase as biorecognition element was constructed for monitoring the degradation of 2,4-DCP, the result indicated that the biosensor was precise and sensitive.
采用 TiO2/UV 光催化、漆酶以及同时光催化-酶处理的方法去除 2,4-二氯苯酚（2,4-DCP）。由于漆酶的快速失活，天然漆酶与 TiO2/UV 的偶联表现出负协同效应。将漆酶共价固定在可控孔玻璃（CPG）上可以有效提高漆酶对 TiO2/UV 诱导失活的稳定性。通过将 CPG-漆酶与 TiO2/UV 偶联，与单独使用固定化漆酶或 TiO2/UV 相比，2,4-DCP 的降解效率显著提高。此外，对于高浓度 2,4-DCP 的降解，增强效果更为显著，例如，在 2h 内，耦合降解过程可实现 5mM 2,4-DCP 的 90%去除率。而对于 TiO2/UV 和 CPG-漆酶过程，在 2h 时，2,4-DCP 的去除率仅为 26.5%和 78.1%。通过考虑 2,4-DCP 降解过程中形成的中间产物，采用中间模型分析了降解动力学。因此，该耦合降解过程的高效率为降解高浓度 2,4-DCP 提供了一种新策略。此外，构建了一种使用固定化漆酶作为生物识别元件的热生物传感器来监测 2,4-DCP 的降解，结果表明该生物传感器精确且灵敏。
