利用油脂真菌卷枝毛霉从玉米乙醇糟液中生产增值油和动物饲料。
Value-added oil and animal feed production from corn-ethanol stillage using the oleaginous fungus Mucor circinelloides.
机构信息
Biorenewable Resources and Technology Program, Iowa State University, Ames, IA 50011, USA; Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, USA; Department of Civil, Construction and Environmental Engineering, Iowa State University, Ames, IA 50011, USA.
出版信息
Bioresour Technol. 2012 Mar;107:368-75. doi: 10.1016/j.biortech.2011.12.031. Epub 2011 Dec 14.
This study highlights the potential of oleaginous fungus, Mucor circinelloides in adsorbing/assimilating oil and nutrients in thin stillage (TS), and producing lipid and protein-rich fungal biomass. Fungal cultivation on TS for 2 days in a 6-L airlift bioreactor, resulted in a 92% increase in oil yield from TS, and 20 g/L of fungal biomass (dry) with a lipid content of 46% (g of oil per 100g dry biomass). Reduction in suspended solids and soluble chemical oxygen demand (SCOD) in TS were 95% and 89%, respectively. The polyunsaturated fatty acids in fungal oil were 52% of total lipids. Fungal cells grown on Yeast Malt (YM) broth had a higher concentration of γ-linolenic acid (17 wt.%) than those grown on TS (1.4 wt.%). Supplementing TS with crude glycerol (10%, v/v) during the stationary growth phase led to a further 32% increase (from 46% to 61%) in cellular oil content.
本研究强调了油脂真菌——卷枝毛霉在吸附/同化稀酒糟（TS）中的油脂和养分，并生产富含脂质和蛋白质的真菌生物质方面的潜力。在 6 升气升式生物反应器中，将真菌在 TS 上培养 2 天，使 TS 的产油率增加了 92%，并获得了 20 克/升（干重）的真菌生物质，其脂质含量为 46%（每 100 克干生物质中的油克数）。TS 中的悬浮固体和可溶解性化学需氧量（SCOD）分别减少了 95%和 89%。真菌油中的多不饱和脂肪酸占总脂质的 52%。在酵母麦芽（YM）肉汤中生长的真菌细胞比在 TS 中生长的细胞（1.4 重量%）含有更高浓度的γ-亚麻酸（17 重量%）。在静止生长阶段，向 TS 中添加粗甘油（10%，v/v），可使细胞内油含量进一步增加 32%（从 46%增加到 61%）。
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