Han Xiang-hui, Ye Yi-yi, Guo Bao-feng, Liu Sheng
Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Zhong Xi Yi Jie He Xue Bao. 2012 Jan;10(1):67-75. doi: 10.3736/jcim20120111.
To investigate the effects of platycodin D in combination with different active ingredients of Chinese herbs under different therapeutic principles on proliferation and invasion of 4T1 and MDA-MB-231 breast cancer cell lines.
The effective doses of platycodin D, Ophiopogon total saponins, curcumenol and osthole in inhibiting proliferation of breast cancer cell lines 4T1 and MDA-MB-231 were detected by methyl thiazolyl tetrazolium (MTT) assay, respectively. Optimized combinations of platycodin D with Ophiopogon total saponins, curcumenol, or osthole were determined by uniform design method. Effects of the optimized combinations of platycodin D with the three ingredients on proliferation and invasion of 4T1 and MDA-MB-231 cells were verified and evaluated by MTT assay and Transwell chamber test, respectively.
Verifying study showed that the inhibitory effects of platycodin D in combination with curcumenol or osthole on proliferation of 4T1 and MDA-MB-231 cells were better than those of platycodin D in combination with Ophiopogon total saponins and each ingredient used alone (P<0.05 or P<0.01). The inhibitory effect of platycodin D in combination with Ophiopogon total saponins or osthole on invasion of 4T1 cells was significantly better than those of platycodin D in combination with curcumenol and each ingredient used alone (P<0.05 or P<0.01). Moreover, the inhibitory effect of platycodin D in combination with curcumenol or osthole on invasion of MDA-MB-231 cells was significantly better than that of platycodin D in combination with Ophiopogon total saponins (P<0.01).
The optimized combinations of platycodin D with three different active ingredients of Chinese herbs under different therapeutic principles can significantly inhibit the proliferation and decrease the invasion of 4T1 and MDA-MB-231 cells. Different platycodin D combinations have different potency in suppressing breast cancer cell proliferation and invasion.
研究桔梗皂苷D与不同治则下的中药活性成分联合应用对4T1和MDA-MB-231乳腺癌细胞系增殖和侵袭的影响。
分别采用甲基噻唑基四氮唑蓝（MTT）法检测桔梗皂苷D、麦冬总皂苷、莪术烯醇和蛇床子素对乳腺癌细胞系4T1和MDA-MB-231增殖的有效抑制剂量。采用均匀设计法确定桔梗皂苷D与麦冬总皂苷、莪术烯醇或蛇床子素的优化组合。分别通过MTT法和Transwell小室实验验证并评估桔梗皂苷D与这三种成分的优化组合对4T1和MDA-MB-231细胞增殖和侵袭的影响。
验证性研究表明，桔梗皂苷D与莪术烯醇或蛇床子素联合应用对4T1和MDA-MB-231细胞增殖的抑制作用优于桔梗皂苷D与麦冬总皂苷联合应用及各单味药单独使用（P<0.05或P<**0.01）。桔梗皂苷D与麦冬总皂苷或蛇床子素联合应用对4T1细胞侵袭的抑制作用明显优于桔梗皂苷D与莪术烯醇联合应用及各单味药单独使用（P<0.05或P<**0.01）。此外，桔梗皂苷D与莪术烯醇或蛇床子素联合应用对MDA-MB-231细胞侵袭的抑制作用明显优于桔梗皂苷D与麦冬总皂苷联合应用（P<**0.01）。
桔梗皂苷D与不同治则下的三种中药活性成分的优化组合可显著抑制4T1和MDA-MB-231细胞的增殖并降低其侵袭能力。不同的桔梗皂苷D组合在抑制乳腺癌细胞增殖和侵袭方面具有不同的效力。
