案例研究：从保存在古粪便中的毛发中提取出古代树懒的DNA。

Case study: ancient sloth DNA recovered from hairs preserved in paleofeces.

作者信息

Clack Andrew A, Macphee Ross D E, Poinar Hendrik N

机构信息

McMaster Ancient DNA Center, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4L9.

出版信息

Methods Mol Biol. 2012;840:51-6. doi: 10.1007/978-1-61779-516-9_7.

DOI:10.1007/978-1-61779-516-9_7

PMID:22237521

Abstract

Ancient hair, which has proved to be an excellent source of well-preserved ancient DNA, is often preserved in paleofeces. Here, we separate and wash hair shafts preserved in a paleofecal specimen believed to be from a Darwin's ground sloth, Mylodon darwinii. After extracting DNA from the recovered and cleaned hair using a protocol optimized for DNA extraction from keratinous substrates, we amplify 12S and 16S rDNA sequences from the DNA extract. As expected, the recovered sequences most closely match previously published sequences of M. darwinii. Our results demonstrate that hair preserved in paleofeces, even from temperate cave environments, is an effective source of ancient DNA.

摘要

已被证明是保存完好的古代DNA极佳来源的古毛发，常常保存在古粪便中。在此，我们从一个据信来自达尔文地懒（Mylodon darwinii）的古粪便标本中分离并清洗了保存下来的毛发轴。在用针对从角质底物中提取DNA优化的方案从回收并清理后的毛发中提取DNA后，我们从DNA提取物中扩增了12S和16S rDNA序列。不出所料，回收的序列与先前发表的达尔文地懒的序列最为匹配。我们的结果表明，保存在古粪便中的毛发，即使来自温带洞穴环境，也是古代DNA的有效来源。